Basic Vector Information
- Vector Name:
- pNZ5319
- Antibiotic Resistance:
- Chloramphenicol
- Length:
- 3737 bp
- Type:
- Mutagenesis vector
- Replication origin:
- p15A ori
- Source/Author:
- Lambert JM, Bongers RS, Kleerebezem M.
pNZ5319 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pNZ5319 vector Sequence
LOCUS 40924_33702 3737 bp DNA circular SYN 18-DEC-2018
DEFINITION Mutagenesis vector pNZ5319, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3737)
AUTHORS Lambert JM, Bongers RS, Kleerebezem M.
TITLE Cre-lox-based system for multiple gene deletions and
selectable-marker removal in Lactobacillus plantarum
JOURNAL Appl. Environ. Microbiol. 73 (4), 1126-1135 (2007)
PUBMED 17142375
REFERENCE 2 (bases 1 to 3737)
AUTHORS Lambert JM, Bongers RS, Kleerebezem M.
TITLE Direct Submission
JOURNAL Submitted (27-JUN-2005) Health and Safety Department, NIZO Food
Research, Kernhemseweg 2, Ede, Gld 6718ZB, The Netherlands
REFERENCE 3 (bases 1 to 3737)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 3737)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
Environ. Microbiol."; date: "2007"; volume: "73"; issue: "4"; pages:
"1126-1135"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(27-JUN-2005) Health and Safety Department, NIZO Food Research,
Kernhemseweg 2, Ede, Gld 6718ZB, The Netherlands"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..3737
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 1..648
/gene="cat"
/label=cat
/note="Chloramphenicol acetyltransferase from
Staphylococcus aureus. Accession#: P00485"
protein_bind 772..805
/label=lox71
/note="Left element (LE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
regulatory complement(949..1050)
/label=Tlas
/note="Tlas"
/regulatory_class="terminator"
CDS complement(1165..1899)
/gene="ermBP"
/label=ermBP
/note="rRNA adenine N-6-methyltransferase from Enterococcus
faecalis. Accession#: P0A4D5"
rep_origin 2328..2873
/direction=RIGHT
/label=p15A ori
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin."
regulatory 3285..3311
/label=TpepN
/note="TpepN"
/regulatory_class="terminator"
protein_bind 3505..3538
/label=lox66
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
regulatory 3609..3721
/label=P32
/note="P32"
/regulatory_class="promoter"
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