pnYGW vector (V004245)

Basic Vector Information

      • Vector Name:
      • pnYGW
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 6516 bp
      • Type:
      • Gateway vector
      • Replication origin:
      • ori
      • Source/Author:
      • Tanaka Y, Nakagawa T.

pnYGW vector Vector Map

pnYGW6516 bp300600900120015001800210024002700300033003600390042004500480051005400570060006300CAP binding sitelac promoterlac operatorM13 revCaMV 35S promoterVN173attR1lac UV5 promoterCmRccdBattR2NOS terminatorM13 fwdf1 oriAmpR promoterAmpRori

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pnYGW vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_33687        6516 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway vector pnYGW DNA, complete sequence.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6516)
  AUTHORS   Tanaka Y, Nakagawa T.
  TITLE     Gateway Vectors for transient expression
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6516)
  AUTHORS   Tanaka Y, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-APR-2011) Contact:Tsuyoshi Nakagawa Shimane 
            University, Center for Integrated Research in Science; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 6516)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6516)
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-APR-2011) Contact:Tsuyoshi Nakagawa Shimane University, Center 
            for Integrated Research in Science; 1060 Nishikawatsu, Matsue, 
            Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pUC119.
FEATURES             Location/Qualifiers
     source          1..6516
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
     promoter        745..1090
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
     CDS             1111..1629
                     /note="N-terminal fragment of mVenus for use in bimolecular
                     fluorescence complementation (BiFC) (Kodama and Hu, 2010)"
     protein_bind    1642..1766
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1803..1833
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1887..2543
                     /note="chloramphenicol acetyltransferase"
     CDS             2888..3190
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(3234..3358)
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3384..3636
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3645..3661)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
     rep_origin      3874..4329
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4611..4715
                     /label=AmpR promoter
     CDS             4716..5573
     rep_origin      5747..6335
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

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