Basic Vector Information
- Vector Name:
- pNV19
- Antibiotic Resistance:
- Kanamycin
- Length:
- 4411 bp
- Type:
- Shuttle vector
- Replication origin:
- ori
- Source/Author:
- Chiba K, Hoshino Y, Ishino K, Kogure T, Mikami Y, Uehara Y, Ishikawa J.
pNV19 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pNV19 vector Sequence
LOCUS 40924_33662 4411 bp DNA circular SYN 18-DEC-2018 DEFINITION Shuttle vector pNV19 DNA, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4411) AUTHORS Chiba K, Hoshino Y, Ishino K, Kogure T, Mikami Y, Uehara Y, Ishikawa J. TITLE Construction of a pair of practical Nocardia-Escherichia coli shuttle vectors JOURNAL Jpn. J. Infect. Dis. 60 (1), 45-47 (2007) PUBMED 17314425 REFERENCE 2 (bases 1 to 4411) AUTHORS Ishikawa J, Chiba K. TITLE Direct Submission JOURNAL Submitted (27-JUL-2006) Contact:Jun Ishikawa National Institute of Infectious Diseases, Department of Bioactive Molecules; 1-23-1 Toyama, Shinjuku, Tokyo 162-8640, Japan URL :http://nocardia.nih.go.jp/ REFERENCE 3 (bases 1 to 4411) TITLE Direct Submission REFERENCE 4 (bases 1 to 4411) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Jpn. J. Infect. Dis."; date: "2007"; volume: "60"; issue: "1"; pages: "45-47" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (27-JUL-2006) Contact:Jun Ishikawa National Institute of Infectious Diseases, Department of Bioactive Molecules; 1-23-1 Toyama, Shinjuku, Tokyo 162-8640, Japan URL :http://nocardia.nih.go.jp/" COMMENT SGRef: number: 3; type: "Journal Article" COMMENT On Dec 18, 2007 this sequence version replaced AB267086.1. FEATURES Location/Qualifiers source 1..4411 /mol_type="other DNA" /organism="synthetic DNA construct" primer_bind 152..168 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" misc_feature 169..225 /label=MCS /note="pUC18/19 multiple cloning site" primer_bind complement(238..254) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(262..278) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(286..316) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(331..352) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(640..1228) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1515..2306) /codon_start=1 /label=NeoR/KanR /note="aminoglycoside phosphotransferase" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" misc_feature 2662..4411 /label=derived from pAL5000 /note="derived from pAL5000" CDS complement(2746..3093) /codon_start=1 /gene="repB" /product="replication protein RepB" /label=repB /protein_id="BAF02360.2" /translation="MSDGYSDGYNRQPTVRKKRRVTAAEGARITGLSERHVVRLVAQER SEWLAEQAARRERIRAYHDDEGHSWPQTAKHFGLHLDTVKRLGYRARKERAAEQEAAQK AHNEADNPPLF" gene complement(2746..3093) /gene="repB" /label=repB CDS complement(3090..4016) /codon_start=1 /gene="repA" /product="replication protein RepA" /label=repA /protein_id="BAF02361.1" /translation="MSHVADEFEQLWLPYWPLASDDLLEGIYRQSRASALGRRYIEANP TALANLLVVDVDHPDAALRALSARGSHPLPNAIVGNRANGHAHAVWALNAPVPRTEYAR RKPLAYMAACAEGLRRAVDGDRSYSGLMTKNPGHIAWETEWLHSDLYTLSHIEAELGAN MPPPRWRQQTTYKAAPTPLGRNCALFDSVRLWAYRPALMRIYLPTRNVDGLGRAIYAEC HARNAEFPCNDVCPGPLPDSEVRAIANSIWRWITTKSRIWADGIVVYEATLSARQSAIS RKGAAARTAASTVARRAKSASAMEALL" gene complement(3090..4016) /gene="repA" /label=repA
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