pNTT104 vector (V004257)

LOCUS       40924_33617        3297 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pNTT104 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Nomura N, Nagase T.
  TITLE     The nucleotide sequence of the pNTT104 vector
  JOURNAL   Published Only in Database (1999)
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Nomura N, Nagase T.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-1999) Nobuo Nomura, Kazusa DNA Research Institute,
            Laboratory of Gene Structure 1; 1532-3 Yana, Kisarazu, Chiba 
            292-0812, Japan (E-mail:nomura@kazusa.or.jp, 
            URL:http://www.kazusa.or.jp, Tel:81-438-52-3932, Fax:81-438-52-3931)
REFERENCE   3  (bases 1 to 3297)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3297)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Published 
            Only in Database (1999)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-AUG-1999) Nobuo Nomura, Kazusa DNA Research Institute, 
            Laboratory of Gene Structure 1"; volume: " 1532-3 Yana, Kisarazu, 
            Chiba 292-0812, Japan (E-mail:nomura@kazusa.or.jp, 
            URL:http://www.kazusa.or.jp, Tel:81-438-52-3932, Fax"; pages: 
            "81-438-52-3931"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     pNTT104
            This sequence is derived from the pUC118 sequence mainly compiled by
            changing the polylinker sequence (sequence at the position between 
            231 and 291 of the pUC118) to the 194-bp sequence (sequence at the 
            position between 231 and 426 of the pNTT104) containing multiple 
            restriction enzyme recognition sites. In addition to that, both the 
            ScaI and NarI sites of the pUC118 were eliminated by nucleotide 
            exchanges at the positions 586 (C to T) and 1802 (G to A) of the 
            pNTT104 sequence, respectively.
FEATURES             Location/Qualifiers
     source          1..3297
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(426..442)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      655..1110
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1392..1496
                     /label=AmpR promoter
     CDS             1497..2354
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2528..3116
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"