Basic Vector Information
- Vector Name:
- pNTT104
- Antibiotic Resistance:
- Ampicillin
- Length:
- 3297 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Nomura N, Nagase T.
pNTT104 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pNTT104 vector Sequence
LOCUS 40924_33617 3297 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pNTT104 DNA, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3297)
AUTHORS Nomura N, Nagase T.
TITLE The nucleotide sequence of the pNTT104 vector
JOURNAL Published Only in Database (1999)
REFERENCE 2 (bases 1 to 3297)
AUTHORS Nomura N, Nagase T.
TITLE Direct Submission
JOURNAL Submitted (31-AUG-1999) Nobuo Nomura, Kazusa DNA Research Institute,
Laboratory of Gene Structure 1; 1532-3 Yana, Kisarazu, Chiba
292-0812, Japan (E-mail:nomura@kazusa.or.jp,
URL:http://www.kazusa.or.jp, Tel:81-438-52-3932, Fax:81-438-52-3931)
REFERENCE 3 (bases 1 to 3297)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 3297)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Published
Only in Database (1999)"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(31-AUG-1999) Nobuo Nomura, Kazusa DNA Research Institute,
Laboratory of Gene Structure 1"; volume: " 1532-3 Yana, Kisarazu,
Chiba 292-0812, Japan (E-mail:nomura@kazusa.or.jp,
URL:http://www.kazusa.or.jp, Tel:81-438-52-3932, Fax"; pages:
"81-438-52-3931"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT pNTT104
This sequence is derived from the pUC118 sequence mainly compiled by
changing the polylinker sequence (sequence at the position between
231 and 291 of the pUC118) to the 194-bp sequence (sequence at the
position between 231 and 426 of the pNTT104) containing multiple
restriction enzyme recognition sites. In addition to that, both the
ScaI and NarI sites of the pUC118 were eliminated by nucleotide
exchanges at the positions 586 (C to T) and 1802 (G to A) of the
pNTT104 sequence, respectively.
FEATURES Location/Qualifiers
source 1..3297
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind 107..128
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 143..173
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 181..197
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 205..221
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(426..442)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 655..1110
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 1392..1496
/label=AmpR promoter
CDS 1497..2354
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 2528..3116
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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