pNTKV1907 vector (V004260)

MSDS

Basic Vector Information

Vector Name:: pNTKV1907

Antibiotic Resistance:: Ampicillin

Length:: 5608 bp

Type:: Cloning vector

Replication origin:: ori

Source/Author:: Skinner H, Pearce M.

Promoter:: mPGK

pNTKV1907 vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pNTKV1907 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_33602        5608 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pNTKV1907, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5608)
  AUTHORS   Skinner H, Pearce M.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-SEP-1998) Technical Services, Stratagene Cloning 
            Systems, 11011 North Torrey Pines Road, La Jolla, CA 92037, USA
REFERENCE   2  (bases 1 to 5608)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5608)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (15-SEP-1998) Technical Services, Stratagene Cloning Systems, 11011 
            North Torrey Pines Road, La Jolla, CA 92037, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5608
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        236..298
                     /label=Mini-TK promoter
                     /note="minimal herpes simplex virus (HSV) thymidine kinase 
                     promoter"
     5'UTR           320..359
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             377..1504
                     /label=HSV TK
                     /note="herpes simplex virus thymidine kinase"
     polyA_signal    1549..1597
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     2027..2043
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    complement(2133..2357)
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(2398..3198)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
     promoter        complement(3212..3712)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     regulatory      3789..3798
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        3842..3946
                     /label=AmpR promoter
     CDS             3947..4804
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4978..5566
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

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