Basic Vector Information
- Vector Name:
- pNR.CFH
- Antibiotic Resistance:
- Kanamycin
- Length:
- 8102 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Host:
- Yeast
- Source/Author:
- Jackson HO, Bibby TS, Moore MC, Terry MJ.
- Promoter:
- URA3
pNR.CFH vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pNR.CFH vector Sequence
LOCUS 40924_33492 8102 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pNR.CFH, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8102)
AUTHORS Jackson HO, Bibby TS, Moore MC, Terry MJ.
TITLE Characterization of constitutive and inducible promoters in
Nannochloropsis gaditana (unpublished)
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 8102)
AUTHORS Jackson HO, Bibby TS, Moore MC, Terry MJ.
TITLE Direct Submission
JOURNAL Submitted (21-FEB-2017) Ocean and Earth Sciences, National
Oceanography Centre, Southampton, European Way, Southampton SO14
3ZH, England
REFERENCE 3 (bases 1 to 8102)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 8102)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(21-FEB-2017) Ocean and Earth Sciences, National Oceanography
Centre, Southampton, European Way, Southampton SO14 3ZH, England"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..8102
/mol_type="other DNA"
/organism="synthetic DNA construct"
regulatory 1..1319
/label=NR promoter
/note="NR promoter"
/regulatory_class="promoter"
misc_feature 1323..1330
/label=NotI restriction site
/note="NotI restriction site"
misc_feature 1342..1349
/label=AscI restriction site
/note="AscI restriction site"
CDS 1356..1379
/label=HRV 3C site
/note="recognition and cleavage site for human rhinovirus
3C and PreScission proteases"
CDS 1386..1409
/label=FLAG
/note="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
CDS 1419..1445
/label=9xHis
/note="9xHis affinity tag"
regulatory 1452..1959
/label=ATPa terminator
/note="ATPa terminator"
/regulatory_class="terminator"
regulatory 1990..2682
/label=UEP promoter
/note="UEP promoter"
/regulatory_class="promoter"
CDS 2689..3708
/label=HygR
/note="aminoglycoside phosphotransferase from E. coli"
terminator 3743..3995
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
primer_bind complement(4029..4045)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(4053..4069)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4077..4107)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(4122..4143)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin 4184..4772
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
rep_origin 4878..5860
/label=2u ori
/note="yeast 2u plasmid origin of replication"
CDS complement(6007..6807)
/label=URA3
/note="orotidine-5'-phosphate decarboxylase, required for
uracil biosynthesis"
promoter complement(6808..7028)
/label=URA3 promoter
CDS complement(7150..7941)
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
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