pNR-46124 vector (V004282)

MSDS

Basic Vector Information

Vector Name:: pNR-46124

Antibiotic Resistance:: Ampicillin

Length:: 7788 bp

Type:: Shuttle vector

Replication origin:: ori

Source/Author:: Charpentier E, Anton AI, Barry P, Alfonso B, Fang Y, Novick RP.

pNR-46124 vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pNR-46124 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_33482        7788 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pNR-46124, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7788)
  AUTHORS   Charpentier E, Anton AI, Barry P, Alfonso B, Fang Y, Novick RP.
  TITLE     Novel cassette-based shuttle vector system for gram-positive 
            bacteria
  JOURNAL   Appl. Environ. Microbiol. 70 (10), 6076-6085 (2004)
  PUBMED    15466553
REFERENCE   2  (bases 1 to 7788)
  AUTHORS   Riojas MA, Bojja K, Hazbon MH.
  TITLE     pNR-46124, A Shuttle Vector for Gram-Positive Bacteria
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 7788)
  AUTHORS   Riojas MA, Bojja K, Hazbon MH.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2014) Bacteriology, BEI Resources/American Type 
            Culture Collection (ATCC), 10801 University Blvd., Manassas, VA 
            20110, USA
REFERENCE   4  (bases 1 to 7788)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 7788)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2004"; volume: "70"; issue: "10"; 
            pages: "6076-6085"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (17-JUN-2014) Bacteriology, BEI Resources/American Type Culture 
            Collection (ATCC), 10801 University Blvd., Manassas, VA 20110, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0.2
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7788
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    7..2589
                     /note="tetM; derived from M21136.1 S.aureus tetracycline 
                     resistance (tetM) gene"
     CDS             295..2229
                     /codon_start=1
                     /gene="tetM"
                     /product="tetracycline resistance protein"
                     /label=tetM
                     /protein_id="AIL56505.1"
                     /translation="MEENHMKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGT
                     TRTDNTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLI
                     SAKDGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKV
                     ELYPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLY
                     HGSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQ
                     RKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKV
                     QMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVST
                     PADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQLK
                     NNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSRIDK
                     VRYMFNKIT"
     gene            295..2229
                     /gene="tetM"
                     /label=tetM
     promoter        2853..2957
                     /label=AmpR promoter
     CDS             2958..3815
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3989..4577
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4759..4775)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             5605..6549
                     /codon_start=1
                     /gene="repC"
                     /product="replication initiation protein"
                     /label=repC
                     /protein_id="AIL56507.1"
                     /translation="MYKNNHANHSNHLENHDLDNFSKTGYSNSRLDAHTVCISDPKLSF
                     DAMTIVGNLNRDNAQALSKFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADSWD
                     RRNMRIEFNPNKLTRDEMIWLKQNIISYMEDDGFTRLDLAFDFEDDLSDYYAMSDKAVK
                     KTIFYGRNGKPETKYFGVRDSNRFIRIYNKKQERKDNADAEVMSEHLWRVEIELKRDMV
                     DYWNDCFSDLHILQPDWKTIQRTADRAIVFMLLSDEEEWGKLHRNSRTKYKNLIKEISP
                     VDLTDLMKSTLKANEKQLQKQIDFWQHEFKFWK"
     gene            5605..6549
                     /gene="repC"
                     /label=repC
     mobile_element  complement(6982..7749)
                     /label=IS1
                     /note="prokaryotic transposable element"

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