pNOV052 vector (V004292)

MSDS

Basic Vector Information

Vector Name:: pNOV052

Antibiotic Resistance:: Gentamycin

Length:: 8181 bp

Type:: Cloning vector

Replication origin:: ori

Source/Author:: Jones AK, Woods AL, Takeoka KT, Shen X, Wei J-R., Caughlan RE, Dean CR.

pNOV052 vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pNOV052 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_33437        8181 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pNOV052, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8181)
  AUTHORS   Jones AK, Woods AL, Takeoka KT, Shen X, Wei J-R., Caughlan RE, Dean 
            CR.
  TITLE     Determinants of antibacterial spectrum and resistance potential of 
            the elongation factor G inhibitor argyrin B in key Gram negative 
            pathogens
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8181)
  AUTHORS   Wei J-R.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2016) Infectious Diseases, Bacteriology, Novartis 
            Institutes for Biomedical Research, 5300 Chiron Way, Emeryville, CA 
            94608, USA
REFERENCE   3  (bases 1 to 8181)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8181)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-OCT-2016) Infectious Diseases, Bacteriology, Novartis Institutes
            for Biomedical Research, 5300 Chiron Way, Emeryville, CA 94608, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8181
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      7..1337
     CDS             complement(1474..2553)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(2554..2631)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        2861..2889
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    2897..2913
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             2936..5056
                     /codon_start=1
                     /gene="fusA"
                     /product="elongation factor G"
                     /label=fusA
                     /protein_id="APS85739.1"
                     /translation="MARTTPINRYRNIGICAHVDAGKTTTTERVLFYTGVNHKLGEVHD
                     GAATTDWMVQEQERGITITSAAVTTFWKGSRGQYDNYRVNVIDTPGHVDFTIEVERSLR
                     VLDGAVVVFCGTSGVEPQSETVWRQANKYGVPRIVYVNKMDRQGANFLRVVEQIKKRLG
                     HTPVPVQLAIGAEENFVGQVDLIKMKAIYWNDDDKGMTYREEEIPAELKDLAEEWRSSM
                     VEAAAEANEELMNKYLEEGELSEAEIKEGLRLRTLACEIVPAVCGSSFKNKGVPLVLDA
                     VIDYLPAPTEIPAIKGVSPDDETVEDERHADDNEPFSSLAFKIATDPFVGTLTFARVYS
                     GVLSSGDSVLNSVKGKKERVGRMVQMHANQREEIKEVRAGDIAALIGMKDVTTGDTLCS
                     IEKPIILERMDFPEPVISVAVEPKTKADQEKMGIALGKLAQEDPSFRVKTDEESGQTII
                     SGMGELHLDIIVDRMKREFGVEANIGKPQVAYRETITKDNVEIEGKFVRQSGGRGQFGH
                     CWIRFSAADVDEKGNITEGLVFENEVVGGVVPKEYIPAIQKGIEEQMKNGVVAGYPLIG
                     LKATVFDGSYHDVDSNEMAFKIAASMATKQLAQKGGGKVLEPIMKVEVVTPEDYMGDVM
                     GDLNRRRGLIQGMEDTVSGKVIRAEVPLGEMFGYATDVRSMSQGRASYSMEFSKYAEAP
                     SNIVEALVKKQG"
     gene            2936..5056
                     /gene="fusA"
                     /label=fusA
     primer_bind     complement(5066..5082)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      5342..5428
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      5520..5547
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        5579..5607
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             5796..6326
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     rep_origin      6351..6806
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     rep_origin      6917..7505
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(7691..7831)
                     /label=bom
                     /note="basis of mobility region from pBR322"

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