Basic Vector Information
- Vector Name:
- pNOV043
- Antibiotic Resistance:
- Gentamycin
- Length:
- 11403 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Wei J-R.
- Promoter:
- Pc
pNOV043 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pNOV043 vector Sequence
LOCUS 40924_33427 11403 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pNOV043, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11403)
AUTHORS Wei J-R.
TITLE LpxK is essential for growth of Acinetobacter baumannii ATCC 19606:
relationship to toxic accumulation of lipid A pathway intermediates
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 11403)
AUTHORS Wei J-R.
TITLE Direct Submission
JOURNAL Submitted (11-APR-2017) Infectious Diseases, Bacteriology, Novartis
Institutes for Biomedical Research, 5300 Chiron Way, Emeryville, CA
94608, USA
REFERENCE 3 (bases 1 to 11403)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 11403)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(11-APR-2017) Infectious Diseases, Bacteriology, Novartis Institutes
for Biomedical Research, 5300 Chiron Way, Emeryville, CA 94608, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..11403
/mol_type="other DNA"
/organism="synthetic DNA construct"
regulatory 7..1337
/regulatory_class="replication_regulatory_region"
regulatory 1430..1629
/regulatory_class="promoter"
CDS 1630..2640
/codon_start=1
/gene="lpxK"
/product="tetraacyldisaccharide 4'-kinase"
/EC_number="2.7.1.130"
/label=lpxK
/note="LpxK; involved in lipid A biosynthesis"
/protein_id="ASL05635.1"
/translation="MSLAQLIQNAWNKQSSWLIVLRPLSCLYRAGFLLNRGFYSSGFKK
VYTAPVPVMVIGNITVGGSGKTPLLIELVNYLKQHNVKVGVISRGYGGSGPFPMLVTSA
SQAAEAGDEPALIVQSTGVPMAVGPNRQAAIELLLASTKLDLIISDDGLQHWALGRQIE
WIVLDQNRGLGNRKLLPEGYLREPVERLKTSTVIEHTFTPTTTLHMHLDAGQPYLLNPS
SATELSFNIQNNYHAVVGIGFPQRFYQTLKGLGVKQFQEHAFRDHHDYSIDDLLFNDDQ
PIITTEKDAVKLLPLLEKHPEFKRPIWVVPVKAVLSTECYQVLNQQLQKLDIQIS"
gene 1630..2640
/gene="lpxK"
/label=lpxK
promoter 8801..8829
/label=Pc promoter
/note="class 1 integron promoter"
CDS 9018..9548
/label=GmR
/note="gentamycin acetyltransferase"
rep_origin 9573..10000
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
rep_origin 10139..10727
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature complement(10913..11053)
/label=bom
/note="basis of mobility region from pBR322"
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