Basic Vector Information
- Vector Name:
- pNOR10-20
- Antibiotic Resistance:
- Chloramphenicol
- Length:
- 5027 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Anderson JC, Voigt CA, Arkin AP.
- Promoter:
- araBAD
pNOR10-20 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pNOR10-20 vector Sequence
LOCUS 40924_33417 5027 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pNOR10-20, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5027)
AUTHORS Anderson JC, Voigt CA, Arkin AP.
TITLE Environmental signal integration by a modular AND gate
JOURNAL Mol. Syst. Biol. 3, 133 (2007)
PUBMED 17700541
REFERENCE 2 (bases 1 to 5027)
AUTHORS Salis HM, Mirsky EA, Voigt CA.
TITLE Automated design of synthetic ribosome binding sites to control
protein expression
JOURNAL Nat. Biotechnol. 27 (10), 946-950 (2009)
PUBMED 19801975
REFERENCE 3 (bases 1 to 5027)
AUTHORS Tamsir A, Tabor JJ, Voigt CA.
TITLE Robust multicellular computing using genetically encoded NOR gates
and chemical 'wires'
JOURNAL Nature 469 (7329), 212-215 (2011)
PUBMED 21150903
REFERENCE 4 (bases 1 to 5027)
AUTHORS Moser F, Voigt CA.
TITLE Genetic Circuit Performance under Conditions Relevant for Industrial
Bioreactors
JOURNAL Unpublished
REFERENCE 5 (bases 1 to 5027)
AUTHORS Anderson JC, Moser F, Tamsir A, Salis H.
TITLE Direct Submission
JOURNAL Submitted (06-JAN-2012) Biological Engineering, MIT, 500 Tech
Square, Rm 209D, Cambridge, MA 02139, USA
REFERENCE 6 (bases 1 to 5027)
TITLE Direct Submission
REFERENCE 7 (bases 1 to 5027)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Syst.
Biol. 3, 133 (2007)"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Nat.
Biotechnol."; date: "2009"; volume: "27"; issue: "10"; pages:
"946-950"
COMMENT SGRef: number: 3; type: "Journal Article"; journalName: "Nature";
date: "2011"; volume: "469"; issue: "7329"; pages: "212-215"
COMMENT SGRef: number: 4; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 5; type: "Journal Article"; journalName: "Submitted
(06-JAN-2012) Biological Engineering, MIT, 500 Tech Square, Rm 209D,
Cambridge, MA 02139, USA"
COMMENT SGRef: number: 6; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..5027
/mol_type="other DNA"
/organism="synthetic DNA construct"
terminator complement(78..121)
/label=bacterial terminator
/note="putative bacterial transcription terminator"
misc_feature 124..145
/label=BioBrick prefix
/note="BioBrick prefix for parts that do not start with
'ATG'"
promoter 145..429
/label=araBAD promoter
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction (Guzman et al., 1995)"
protein_bind 458..476
/label=tet operator
/note="bacterial operator O2 for the tetR and tetA genes"
regulatory 506..511
/label=Ptet BBa_R0040
/note="Ptet BBa_R0040"
/regulatory_class="promoter"
regulatory 520..530
/label=BBa_B0033
/note="BBa_B0033"
/regulatory_class="ribosome_binding_site"
CDS 537..1247
/label=lambda repressor
/note="phage lambda repressor"
CDS 1248..1280
/label=ssrA tag (LVA)
/note="C-terminal peptide that mediates degradation in
bacteria through the ClpXP and ClpAP proteases (McGinness
et al., 2006)"
terminator 1328..1399
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 1415..1442
/label=T7Te terminator
/note="phage T7 early transcription terminator"
regulatory 1455..1489
/label=BBa_J23117
/note="BBa_J23117"
/regulatory_class="promoter"
regulatory 1498..1509
/label=BBa_B0034
/note="BBa_B0034"
/regulatory_class="ribosome_binding_site"
RBS 1498..1509
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 1516..2391
/label=araC
/note="L-arabinose regulatory protein"
CDS 2421..3041
/label=TetR
/note="tetracycline repressor TetR"
misc_feature 3048..3068
/label=BioBrick suffix
/note="universal suffix for all parts"
terminator 3069..3128
/label=his operon terminator
/note="This putative transcriptin terminator from the E.
coli his operon has a 2-bp deletion introduced during
synthesis. Its efficiency has not been determined."
rep_origin complement(3375..3963)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
terminator complement(4051..4145)
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
CDS complement(4169..4825)
/label=CmR
/note="chloramphenicol acetyltransferase"
promoter complement(4826..4928)
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
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