Basic Vector Information
- Vector Name:
- pNK207A
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7904 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Kandul NP, Guo M, Hay BA.
- Promoter:
- Ac5
pNK207A vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pNK207A vector Sequence
LOCUS 40924_33327 7904 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pNK207A, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7904)
AUTHORS Kandul NP, Guo M, Hay BA.
TITLE A positive readout single transcript reporter for site-specific mRNA
cleavage
JOURNAL PeerJ (2017) In press
REFERENCE 2 (bases 1 to 7904)
AUTHORS Kandul NP, Guo M, Hay BA.
TITLE Direct Submission
JOURNAL Submitted (02-JAN-2017) Biology and Biological Engineering,
California Institute of Technology, 1200 E. California Blvd.,
Pasadena, CA 91125, USA
REFERENCE 3 (bases 1 to 7904)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 7904)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "PeerJ
(2017) In press"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(02-JAN-2017) Biology and Biological Engineering, California
Institute of Technology, 1200 E. California Blvd., Pasadena, CA
91125, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..7904
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin 4..460
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 601..617
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 627..645
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
promoter 699..3208
/label=Ac5 promoter
/note="Drosophila melanogaster actin 5C promoter"
misc_RNA 3239..3336
/product="CopT"
CDS 3361..5007
/label=luciferase
/note="firefly luciferase"
misc_RNA 5285..5374
/product="CopA"
promoter complement(5716..5734)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(5755..5771)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(5779..5795)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5803..5833)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(5848..5869)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(6157..6745)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(6919..7776)
/label=AmpR
/note="beta-lactamase"
promoter complement(7777..7881)
/label=AmpR promoter
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