pNK071 vector (V004303)

MSDS

Basic Vector Information

Vector Name:: pNK071

Antibiotic Resistance:: Ampicillin

Length:: 6352 bp

Type:: Cloning vector

Replication origin:: ori

Source/Author:: Kandul NP, Guo M, Hay BA.

Promoter:: Ac5

pNK071 vector Vector Map

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Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pNK071 vector Sequence

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Download GeneBank File(.gb)

LOCUS       40924_33317        6352 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pNK071, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6352)
  AUTHORS   Kandul NP, Guo M, Hay BA.
  TITLE     A positive readout single transcript reporter for site-specific mRNA
            cleavage
  JOURNAL   PeerJ (2017) In press
REFERENCE   2  (bases 1 to 6352)
  AUTHORS   Kandul NP, Guo M, Hay BA.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JAN-2017) Biology and Biological Engineering, 
            California Institute of Technology, 1200 E. California Blvd., 
            Pasadena, CA 91125, USA
REFERENCE   3  (bases 1 to 6352)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6352)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PeerJ 
            (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-JAN-2017) Biology and Biological Engineering, California 
            Institute of Technology, 1200 E. California Blvd., Pasadena, CA 
            91125, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2565
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2606..3301
                     /label=mKate2
                     /note="monomeric far-red fluorescent protein (Shcherbo et
                     al., 2009)"
     intron          3319..3598
     ncRNA           3337..3548
                     /label=miRNA
                     /note="miR-6.1 backbone with a synthetic guide miRNA"
                     /ncRNA_class="miRNA"
     ncRNA           3458..3479
                     /label=other
                     /note="synthetic guide miRNA"
                     /ncRNA_class="other"
     CDS             3637..3678
                     /label=V5 tag
                     /note="epitope tag from simian virus 5"
     CDS             3688..3705
                     /label=6xHis
                     /note="6xHis affinity tag"
     polyA_signal    complement(3778..3912)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4534..5122)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5296..6153)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6154..6258)
                     /label=AmpR promoter

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