NGFR vector (V004304)

Basic Vector Information

      • Vector Name:
      • NGFR
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 6522 bp
      • Type:
      • Mammalian Expression, Retroviral
      • Copy Number:
      • High Copy
      • Cloning Method:
      • Restriction Enzyme
      • 5' Primer:
      • pLXSN 5'

NGFR vector Vector Map

NGFR6522 bp300600900120015001800210024002700300033003600390042004500480051005400570060006300pBR322ori-FAmpRAmpR promoterpBRforEcopGEX 3'pRS-markerIn lacZ genepBRrevBam5' LTRMESV Psigag (truncated)IRES3' LTRlac operatorlac promoterCAP binding siteL4440ori

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

NGFR vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                6522 bp ds-DNA     circular SYN 08-JUN-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    NGFR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6522)
  AUTHORS   Izon DJ, Punt JA, Xu L, Karnell FG, Allman D, Myung PS, Boerth NJ, 
            Pui JC, Koretzky GA, Pear WS
  TITLE     Notch1 regulates maturation of CD4+ and CD8+ thymocytes by 
            modulating TCR signal strength.
  JOURNAL   Immunity. 2001 Mar . 14(3):253-64.
  PUBMED    11290335
REFERENCE   2  (bases 1 to 6522)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Immunity. 
            2001 Mar . 14(3):253-64."
FEATURES             Location/Qualifiers
     source          1..6522
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(2..21)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(681..1541)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1304..1323
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1542..1646)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1714..1732
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1770..1792)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1892..1911
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2105..2127
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2255..2274
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             2474..2990
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    3054..3395
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             3462..3878
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     3808..3830
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     3846..3862
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    3924..4476
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4068..4085)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4295..4314
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     LTR             5390..5904
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    6066..6082
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6090..6120)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6135..6156
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6273..6290)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(6444..6522,1..510))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

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