Basic Vector Information
- Vector Name:
- pND707
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4284 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Love CA, Lilley PE, Dixon NE.
pND707 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pND707 vector Sequence
LOCUS 40924_33113 4284 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pND707 DNA, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4284)
AUTHORS Love CA, Lilley PE, Dixon NE.
TITLE Stable high-copy-number bacteriophage lambda promoter vectors for
overproduction of proteins in Escherichia coli
JOURNAL Gene 176 (1-2), 49-53 (1996)
PUBMED 8918231
REFERENCE 2 (bases 1 to 4284)
AUTHORS Yasuda S.
TITLE Direct Submission
JOURNAL Submitted (07-FEB-2006) Contact:Seiichi Yasuda National Institute of
Genetics, Microbial Genetics; Yata 1111, Mishima, Shizuoka 411-8540,
Japan
REFERENCE 3 (bases 1 to 4284)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 4284)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Gene 176
(1-2), 49-53 (1996)"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(07-FEB-2006) Contact:Seiichi Yasuda National Institute of Genetics,
Microbial Genetics; Yata 1111, Mishima, Shizuoka 411-8540, Japan"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..4284
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 53..419
/label=partition(pSC101)
/note="partition(pSC101)"
promoter 474..578
/label=AmpR promoter
CDS 579..1436
/label=AmpR
/note="beta-lactamase"
rep_origin 1610..2198
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 2486..2507
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2522..2552
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2560..2576
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2584..2600
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
CDS complement(2859..3569)
/label=lambda repressor
/note="phage lambda repressor"
regulatory 3617..3644
/label=lambda PR
/note="lambda PR"
/regulatory_class="promoter"
RBS 3658..3666
/label=Shine-Dalgarno sequence
/note="full consensus sequence for ribosome-binding sites
upstream of start codons in E. coli; complementary to a
region in the 3' end of the 16S rRNA (Chen et al., 1994)"
regulatory 3831..3859
/label=lambda PL
/note="lambda PL"
/regulatory_class="promoter"
RBS 4005..4027
/label=RBS
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
primer_bind complement(4127..4143)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
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