Basic Vector Information
- Vector Name:
- pND50
- Antibiotic Resistance:
- Chloramphenicol
- Length:
- 3772 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Yamagishi J, Kojima T, Oyamada Y, Fujimoto K, Hattori H, Nakamura S, Inoue M.
pND50 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pND50 vector Sequence
LOCUS 40924_33103 3772 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pND50 DNA, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3772)
AUTHORS Yamagishi J, Kojima T, Oyamada Y, Fujimoto K, Hattori H, Nakamura S,
Inoue M.
TITLE Alterations in the DNA topoisomerase IV grlA gene responsible for
quinolone resistance in Staphylococcus aureus
JOURNAL Antimicrob. Agents Chemother. 40 (5), 1157-1163 (1996)
PUBMED 8723458
REFERENCE 2 (bases 1 to 3772)
AUTHORS Inoue R, Kaito C, Tanabe M, Kamura K, Akimitsu N, Sekimizu K.
TITLE Genetic identification of two distinct DNA polymerases, DnaE and
PolC, that are essential for chromosomal DNA replication in
Staphylococcus aureus
JOURNAL Mol. Genet. Genomics 266 (4), 564-571 (2001)
PUBMED 11810227
REFERENCE 3 (bases 1 to 3772)
AUTHORS Matsuo M, Kurokawa K, Nishida S, Li Y, Takimura H, Kaito C, Fukuhara
N, Maki H, Miura K, Murakami K, Sekimizu K.
TITLE Isolation and mutation site determination of the
temperature-sensitive murB mutants of Staphylococcus aureus
JOURNAL FEMS Microbiol. Lett. 222 (1), 107-113 (2003)
PUBMED 12757953
REFERENCE 4 (bases 1 to 3772)
AUTHORS Kurokawa K, Matsuo M, Sekimizu K.
TITLE Direct Submission
JOURNAL Submitted (03-MAR-2005) Kenji Kurokawa, The University of Tokyo,
Graduate School of Pharmaceutical Sciences; 7-3-1, Hongo, Bunkyo-ku,
Tokyo 113-0033, Japan (E-mail:kurokawa@mol.f.u-tokyo.ac.jp,
URL:http://www.f.u-tokyo.ac.jp/~hassei/, Tel:81-3-5841-4821,
Fax:81-3-5684-2973)
REFERENCE 5 (bases 1 to 3772)
TITLE Direct Submission
REFERENCE 6 (bases 1 to 3772)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Antimicrob.
Agents Chemother."; date: "1996"; volume: "40"; issue: "5"; pages:
"1157-1163"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Mol. Genet.
Genomics"; date: "2001"; volume: "266"; issue: "4"; pages: "564-571"
COMMENT SGRef: number: 3; type: "Journal Article"; journalName: "FEMS
Microbiol. Lett."; date: "2003"; volume: "222"; issue: "1"; pages:
"107-113"
COMMENT SGRef: number: 4; type: "Journal Article"; journalName: "Submitted
(03-MAR-2005) Kenji Kurokawa, The University of Tokyo, Graduate
School of Pharmaceutical Sciences"; volume: " 7-3-1, Hongo,
Bunkyo-ku, Tokyo 113-0033, Japan
(E-mail:kurokawa@mol.f.u-tokyo.ac.jp,
URL:http://www.f.u-tokyo.ac.jp/~hassei/, Tel:81-3-5841-4821, Fax";
pages: "81-3-5684-2973"
COMMENT SGRef: number: 5; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..3772
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 1..57
/label=MCS
/note="pUC18/19 multiple cloning site"
rep_origin 264..852
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1430..2077)
/gene="cat"
/label=cat
/note="Chloramphenicol acetyltransferase from
Staphylococcus aureus. Accession#: P00485"
CDS complement(2359..3360)
/label=repB
/note="RepB replication protein"
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