pNBio vector (V004350)

LOCUS       V004350                 6895 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004350
VERSION     V004350
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6895)
  AUTHORS   Kulman JD, Satake M, Harris JE.
  TITLE     A versatile system for site-specific enzymatic biotinylation and
            regulated expression of proteins in cultured mammalian cells
  JOURNAL   Protein Expr. Purif. 52 (2), 320-328 (2007)
   PUBMED   17084093
REFERENCE   2  (bases 1 to 6895)
  AUTHORS   Kulman JD, Satake M, Harris JE.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2006) Biochemistry, University of Washington, N.E.
            Pacific St., Seattle, WA 98195, USA
REFERENCE   3  (bases 1 to 6895)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6895)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein
            Expr. Purif."; date: "2007"; volume: "52"; issue: "2"; pages:
            "320-328"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (28-APR-2006) Biochemistry, University of Washington, N.E. Pacific
            St., Seattle, WA 98195, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6895
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    15..285
                     /label="tetracycline response element"
                     /note="contains seven copies of the tetracycline operator
                     tetO"
     promoter        318..356
                     /label="minimal CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     regulatory      477..485
                     /label="Kozak site"
                     /note="Kozak site"
                     /regulatory_class="ribosome_binding_site"
     regulatory      480..489
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    492..530
                     /label="biotin acceptor peptide (BioTag)-encoding"
                     /note="biotin acceptor peptide (BioTag)-encoding"
     misc_feature    532..551
                     /note="multiple cloning site; MCS"
     misc_feature    652..1238
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     regulatory      1270..1278
                     /gene="birA"
                     /label="Kozak site"
                     /note="Kozak site"
                     /regulatory_class="ribosome_binding_site"
     regulatory      1273..1282
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1282..1305
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             1318..2280
                     /label="BirA"
                     /note="E. coli biotin protein ligase"
     intron          2348..2920
                     /label="beta-globin intron"
                     /note="intron from rabbit beta-globin gene"
     CDS             2924..3046
                     /gene="HBB"
                     /label="Hemoglobin subunit beta"
                     /note="Hemoglobin subunit beta from Colobus guereza.
                     Accession#: Q9TT33"
     polyA_signal    3117..3172
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     rep_origin      complement(3734..4322)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4496..5353)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5354..5458)
                     /label="AmpR promoter"
     promoter        5563..5892
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             5985..6581
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     polyA_signal    6814..6895
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"