pNB102 vector (V004357)

LOCUS       40924_33013        9811 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pNB102, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9811)
  AUTHORS   Bongio NJ, Lampe DJ.
  TITLE     Inhibition of Plasmodium berghei Development in Mosquitoes by 
            Effector Proteins Secreted from Asaia sp. Bacteria Using a Novel 
            Native Secretion Signal
  JOURNAL   PLoS ONE 10 (12), E0143541 (2015)
  PUBMED    26636338
REFERENCE   2  (bases 1 to 9811)
  AUTHORS   Bongio NJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-SEP-2015) Biology, Shenandoah University, 1460 
            University Drive, Winchester, VA 22601, USA
REFERENCE   3  (bases 1 to 9811)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9811)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2015"; volume: "10"; issue: "12"; pages: "E0143541"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-SEP-2015) Biology, Shenandoah University, 1460 University Drive,
            Winchester, VA 22601, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9811
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             458..1249
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      1552..1762
                     /label=nptII
                     /note="nptII"
                     /regulatory_class="promoter"
     CDS             1767..3524
                     /codon_start=1
                     /product="TonB dependent siderophore receptor"
                     /label=TonB dependent siderophore receptor
                     /protein_id="ALR84564.1"
                     /translation="MPRAARRVPGLFPHRLGDAPATSRPETIMVTGSLFRDPNTTSASP
                     ITHITMKDMQQRGIKTVTDALQLLSSNGAGNLTNAWSAGGGFAAGASAPSLRGLSTDST
                     LVLMDGQRLSYYPLADDGERNFVDTNWMPQSIMQGVDVLQDGGSATYGADAVAGVVNMI
                     TRKEIKGFEGNAEGGLTQRGDAGHQRLYATWGHGDLHKDGYNFYINSEYQQDDPLYYRQ
                     LNSPYNNGDLTGIGGSNGNDNIVSGGSITNFAPSTVALVRPVTGTGTNTAPWSLLNQQA
                     GCRGLDGLVTGTVGGSTGISQACSWNTQKYAQVAPDLRRVSATAHFTANVTERSQLTAM
                     FTWSQARSIITGTPYSTYGGTSYSQAKLATAQNTYLPVYLADGSLNPNNPYAAQGEQAQ
                     VNYRFGDLIPTTMDTSQNFRGSVRYNGWHPSRWGSDWNYDVNFVGMNTLLNQTITGVPT
                     ISGIQNAITSGSYNFVDPSQNSQSVLNAIAPRNVINARTQEYSGEATLSKGLFHLPGGM
                     AKLAIGGNIRYEALNDPNANPLDTANPANQYTSYINPVNAQGSRWVESGFFQAEAPIVE
                     QKLISEEDLN"
     CDS             3492..3521
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3534..4418
                     /codon_start=1
                     /product="anti-Pbs21-Shiva1 immunotoxin"
                     /label=anti-Pbs21-Shiva1 immunotoxin
                     /protein_id="ALR84565.1"
                     /translation="SEVQLQQSGAELVRSGASVQLSCTASGFNIKDFYIHWVKQRPEQG
                     LEWIGCIDPESGDVEYAPKFQDKATMTTDTSSNTAYLQLSGLTSGDTAVYFCNALILRF
                     SSAMDYWGRGTSVTVSSTGGGGSGGGGSGGGGSDIDVLMTQTPLSLPISLGDQASISCR
                     SSQSIVHSNGNTYLEWYLQRPGQSPKLLLFKVSNRFSGVPDRFSGSGSGTDFTLKITRV
                     EAEDLGVYYCFQGSHLPYTFGGGTKLEIKRGPGGPMPRWRLFRRIDRVGKQIKQGILRA
                     GPAIALVGDARAVG"
     CDS             4431..5768
                     /codon_start=1
                     /product="alkaline phosphatase"
                     /label=alkaline phosphatase
                     /protein_id="ALR84566.1"
                     /translation="PVLENRAAQGDITAPGGARRLTGDQTAALRDSLSDKPAKNIILLI
                     GDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPDYVTDSAASATA
                     WSTGVKTYNGALGVDIHEKDHPTILEMAKAAGLATGNVSTAELQDATPAALVAHVTSRK
                     CYGPSATSEKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAETATAGEWQGKTLR
                     EQAQARGYQLVSDAASLNSVTEANQQKPLLGLFADGNMPVRWLGPKATYHGNIDKPAVT
                     CTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGASIDKQDHAANPCGQIGETVD
                     LDEAVQRALEFAKKEGNTLVIVTADHAHASQIVAPDTKAPGLTQALNTKDGAVMVMSYG
                     NSEEDSQEHTGSQLRIAAYGPHAANVVGLTDQTDLFYTMKAALGLK"
     CDS             complement(7360..8019)
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(8020..8789)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"