Basic Vector Information
- Vector Name:
- pNB1
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4813 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Host:
- Plants
- Source/Author:
- Budhagatapalli N, Schedel S, Gurushidze M, Pencs S, Hiekel S, Rutten T, Kusch S, Morbitzer R, Lahaye T, Panstruga R, Kumlehn J, Hensel G.
- Promoter:
- CaMV 35S (enhanced)
pNB1 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pNB1 vector Sequence
LOCUS 40924_33008 4813 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pNB1, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4813)
AUTHORS Budhagatapalli N, Schedel S, Gurushidze M, Pencs S, Hiekel S, Rutten
T, Kusch S, Morbitzer R, Lahaye T, Panstruga R, Kumlehn J, Hensel G.
TITLE A simple test for the cleavage activity of customized endonucleases
in plants
JOURNAL Plant Methods 12, 18 (2016)
PUBMED 26962325
REFERENCE 2 (bases 1 to 4813)
AUTHORS Budhagatapalli N, Kumlehn J, Hensel G.
TITLE Direct Submission
JOURNAL Submitted (10-FEB-2016) Physiology and Cell Biology, Leibniz
Institute of Plant Genetics and Crop Plant Research (IPK),
Corrensstr. 3, Stadt Seeland/OT Gatersleben 06466, Germany
REFERENCE 3 (bases 1 to 4813)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 4813)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Plant
Methods 12, 18 (2016)"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(10-FEB-2016) Physiology and Cell Biology, Leibniz Institute of
Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, Stadt
Seeland/OT Gatersleben 06466, Germany"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..4813
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 99..776
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
misc_feature 828..901
/note="sig peptide; derived from Vicia faba legumin B gene
LeB4"
misc_feature 903..926
/label=multiple cloning site
/note="multiple cloning site"
CDS 927..1640
/label=EGFP
/note="enhanced GFP"
terminator 1672..1924
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
promoter complement(1972..1990)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(1997..2013)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin complement(2154..2609)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2636..2740
/label=AmpR promoter
CDS 2741..3598
/label=AmpR
/note="beta-lactamase"
rep_origin 3772..4360
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 4648..4669
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 4684..4714
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4722..4738
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 4746..4762
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
promoter 4783..4801
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
This page is informational only.