Basic Vector Information
- Vector Name:
- pNB-3
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5418 bp
- Type:
- Expression vector
- Replication origin:
- ori
- Source/Author:
- Yang Y, Ma J, Song Z, Wu M.
pNB-3 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pNB-3 vector Sequence
LOCUS 40924_33003 5418 bp DNA circular SYN 18-DEC-2018
DEFINITION Expression vector pNB-3, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5418)
AUTHORS Yang Y, Ma J, Song Z, Wu M.
TITLE HIV-1 TAT-mediated protein transduction and subcellular localization
using novel expression vectors
JOURNAL FEBS Lett. 532 (1-2), 36-44 (2002)
PUBMED 12459459
REFERENCE 2 (bases 1 to 5418)
AUTHORS Yang YH, Wu M.
TITLE Direct Submission
JOURNAL Submitted (29-JUN-2002) Department of Molecular and Cellular
Biology, School of Life Sciences, University of Science and
Technology of China, 96 Jingzai Road, Hefei, Anhui 230026, P.R.
China
REFERENCE 3 (bases 1 to 5418)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 5418)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett.
532 (1-2), 36-44 (2002)"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(29-JUN-2002) Department of Molecular and Cellular Biology, School
of Life Sciences, University of Science and Technology of China, 96
Jingzai Road, Hefei, Anhui 230026, P.R. China"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..5418
/mol_type="other DNA"
/organism="synthetic DNA construct"
terminator complement(26..73)
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
CDS complement(140..157)
/label=6xHis
/note="6xHis affinity tag"
misc_feature 158..211
/note="multiple cloning site; MCS"
RBS complement(222..244)
/label=RBS
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
protein_bind complement(259..283)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(284..302)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
promoter 611..688
/label=lacI promoter
CDS 689..1768
/label=lacI
/note="lac repressor"
protein_bind 1784..1805
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
CDS 2580..2768
/label=rop
/note="Rop protein, which maintains plasmids at low copy
number"
misc_feature 2873..3015
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(3201..3789)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3963..4820)
/label=AmpR
/note="beta-lactamase"
promoter complement(4821..4925)
/label=AmpR promoter
rep_origin complement(4952..5407)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
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