Basic Vector Information
- Vector Name:
- pNASSbeta
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6541 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- MacGregor GR, Caskey CT.
pNASSbeta vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pNASSbeta vector Sequence
LOCUS 40924_32993 6541 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pNASSbeta, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6541)
AUTHORS MacGregor GR, Caskey CT.
TITLE Construction of plasmids that express E. coli beta-galactosidase in
mammalian cells
JOURNAL Nucleic Acids Res. 17 (6), 2365 (1989)
PUBMED 2495524
REFERENCE 2 (bases 1 to 6541)
AUTHORS Kitts PA.
TITLE CLONTECH Vectors On Disc version 1.3
JOURNAL Unpublished
REFERENCE 3 (bases 1 to 6541)
AUTHORS Kitts PA.
TITLE Direct Submission
JOURNAL Submitted (07-OCT-1993) Paul A. Kitts, CLONTECH Laboratories, Inc.,
1020 East Meadow Circle, Palo Alto, CA 94303, USA
REFERENCE 4 (bases 1 to 6541)
TITLE Direct Submission
REFERENCE 5 (bases 1 to 6541)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
Acids Res."; date: "1989"; volume: "17"; issue: "6"; pages: "2365"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
(07-OCT-1993) Paul A. Kitts, CLONTECH Laboratories, Inc., 1020 East
Meadow Circle, Palo Alto, CA 94303, USA"
COMMENT SGRef: number: 4; type: "Journal Article"
COMMENT This vector can be obtained from CLONTECH Laboratories, Inc., 1020
East Meadow Circle, Palo Alto, CA 94303, USA. To place an order call
(415) 424-8222 or (800) 662-2566, extension 1. International
customers, please contact your local distributor. For technical
information, call (415) 424- 8222 or (800) 662-2566, extension 3.
This sequence has been compiled from information in the sequence
databases, published literature and other sources, together with
partial sequences obtained by CLONTECH; this vector has not been
completely sequenced. If you suspect there is an error in this
sequence, please contact CLONTECH's Technical Service Department at
(415) 424-8222 or (800) 662-2566, extension 3 or E-mail
TECH@CLONTECH.COM.
FEATURES Location/Qualifiers
source 1..6541
/mol_type="other DNA"
/organism="synthetic DNA construct"
intron 49..145
/label=SV40 intron
/note="modified SV40 intron with splice donor and acceptor
sites"
CDS 346..3390
/codon_start=1
/label=lacZ
/note="beta-galactosidase"
/translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
SWSPSVSAELQLSAGRYHYQLVWCQK"
polyA_signal complement(3680..3814)
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3925..3941)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(3949..3965)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3973..4003)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(4018..4039)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(4327..4915)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5089..5946)
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5947..6051)
/label=AmpR promoter
primer_bind 6525..6541
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
This page is informational only.