Basic Vector Information
pSC101 ori is a low-copy replication origin that requires the Rep101 protein, which is a temperature-sensitive protein. When bacteria containing a plasmid with this ori is cultured at 37℃, the plasmid will be lost.
- Vector Name:
- pMWO-076
- Antibiotic Resistance:
- Kanamycin
- Length:
- 4492 bp
- Type:
- Expression vector
- Replication origin:
- pSC101 ori
- Source/Author:
- Obrist MW, Miller VL.
- Copy Number:
- Low Copy
- Promoter:
- PLtetO-1
- Growth Temperature:
- 30℃
pMWO-076 vector Map
pMWO-076 vector Sequence
LOCUS 40924_32848 4492 bp DNA circular SYN 18-DEC-2018
DEFINITION Expression vector pMWO-076, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4492)
AUTHORS Obrist MW, Miller VL.
TITLE Low copy expression vectors for use in Yersinia sp. and related
organisms
JOURNAL Plasmid 68 (1), 33-42 (2012)
PUBMED 22445322
REFERENCE 2 (bases 1 to 4492)
AUTHORS Obrist MW, Miller VL.
TITLE Direct Submission
JOURNAL Submitted (14-DEC-2011) Microbiology and Immunology, University of
North Carolina at Chapel Hill, 116 Manning Drive, 505 Mary Ellen
Jones, CB# 7290, Chapel Hill, NC 27599, USA
REFERENCE 3 (bases 1 to 4492)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 4492)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid";
date: "2012"; volume: "68"; issue: "1"; pages: "33-42"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(14-DEC-2011) Microbiology and Immunology, University of North
Carolina at Chapel Hill, 116 Manning Drive, 505 Mary Ellen Jones,
CB# 7290, Chapel Hill, NC 27599, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT ##Assembly-Data-START##
Assembly Method :: DNASTAR Lasergene SeqMan Pro v. 7.2.1 (1) Intel
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..4492
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 60..77
/note="MWO-fw; forward sequencing primer"
promoter 75..148
/label=PLtetO-1 promoter
/note="modified phage lambda PL promoter with tet operator
sites (Lutz and Bujard, 1997)"
misc_feature complement(155..262)
/label=MCS
/note="pBluescript multiple cloning site"
promoter complement(271..289)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(398..420)
/note="MWO-rv-1; reverse sequencing primer"
CDS complement(739..1686)
/label=Rep101
/note="RepA protein needed for replication with the pSC101
origin"
rep_origin complement(1734..1956)
/direction=LEFT
/label=pSC101 ori
/note="low-copy replication origin that requires the Rep101
protein"
terminator complement(2360..2446)
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
CDS complement(2500..3120)
/label=TetR
/note="tetracycline repressor TetR"
CDS complement(3340..4128)
/label=SmR
/note="aminoglycoside adenylyltransferase (Murphy, 1985)"
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