Basic Vector Information
- Vector Name:
- pMV5
- Length:
- 10047 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Inui M, Tsuge Y, Suzuki N, Vertes AA, Yukawa H.
- Promoter:
- sacB
pMV5 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMV5 vector Sequence
LOCUS V004389 10047 bp DNA circular SYN 18-DEC-2018
DEFINITION Exported.
ACCESSION V004389
VERSION V004389
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 10047)
AUTHORS Inui M, Tsuge Y, Suzuki N, Vertes AA, Yukawa H.
TITLE Isolation and characterization of a native composite transposon,
Tn14751, carrying 17.4 kilobases of Corynebacterium glutamicum
chromosomal DNA
JOURNAL Appl. Environ. Microbiol. 71 (1), 407-416 (2005)
PUBMED 15640215
REFERENCE 2 (bases 1 to 10047)
AUTHORS Inui M.
TITLE Direct Submission
JOURNAL Submitted (01-JUL-2004) Masayuki Inui, Research Institute of
Innovative Technology for the Earth (RITE), Microbiology Research
Group; 9-2 Kizugawadai, Kizu, Soraku, Kyoto 619-0292, Japan
(E-mail:inui@rite.or.jp, Tel:81-774-75-2308, Fax:81-774-75-2321)
REFERENCE 3 (bases 1 to 10047)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 10047)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
Environ. Microbiol."; date: "2005"; volume: "71"; issue: "1"; pages:
"407-416"
SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(01-JUL-2004) Masayuki Inui, Research Institute of Innovative
Technology for the Earth (RITE), Microbiology Research Group";
volume: " 9-2 Kizugawadai, Kizu, Soraku, Kyoto 619-0292, Japan
(E-mail:inui@rite.or.jp, Tel:81-774-75-2308, Fax"; pages:
"81-774-75-2321"
SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..10047
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 870..2357
/codon_start=1
/gene="repA"
/product="RepA"
/label="repA"
/protein_id="BAD83816.1"
/translation="MYMIIPNSTPAYPISGSRVNTPSRLRPDCAGLDDTPASTVDRDKL
LAHLGRTALHGSISRNFKGAYRLVVDKETGEKRSVPNLYRIDSEKLGRCEYVMLTSKQY
ASVMVIDVDQIGEAGGHPENLNSYVKGVIWVLVQHGIGPAWAGINPISGKAQFIWLIDP
VYAGKNRASRNMELLKATSHELGELLDHDPHFAHRFSRSPFYTGKSPEAYRWYCQHDRV
IRLQDFLRQVREMAGQSQHIKNKRQQFNSGRELINAVKTRREEAQAFKALAEDVENEIS
EEIDQYDPELIDGVRVRWISQGVAARDETAFSHALKIGHRLRKAGQRLTDAAVIDAYEH
AYNVAQQQGSAGRDSEMPPMRDRQTMARRVRGYVTQSKTNTSLGASAPGRVTSSERKAL
ATMGRKGGQKAAQRWKTDPEGQYAQNQLQKLKKTHRKKRVEGQTTRAKIQALIGEAYVQ
TGEVLTRKQIVDETGLSRATVTRHLAALREQGALPET"
gene 870..2357
/gene="repA"
/label="repA"
regulatory 2067..2076
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
primer_bind complement(4213..4229)
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 4237..4253
/label="lac operator"
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4261..4291)
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind complement(4306..4327)
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(4615..5203)
/direction=LEFT
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5692..5708
/label="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
promoter 5749..6194
/label="sacB promoter"
/note="sacB promoter and control region"
CDS 6195..7613
/label="SacB"
/note="secreted levansucrase that renders bacterial growth
sensitive to sucrose"
CDS 7823..8602
/gene="ant1"
/label="Spectinomycin 9-adenylyltransferase"
/note="Spectinomycin 9-adenylyltransferase from
Staphylococcus aureus (strain N315). Accession#: P0A0D1"
mobile_element complement(8764..9531)
/label="IS1"
/note="prokaryotic transposable element"
primer_bind 10005..10021
/label="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
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