Basic Vector Information
- Vector Name:
- pMU770
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5650 bp
- Type:
- Expression vector
- Replication origin:
- ori
- Source/Author:
- Olson DG, Lynd LR.
pMU770 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMU770 vector Sequence
LOCUS V004404 5650 bp DNA circular SYN 18-DEC-2018
DEFINITION Exported.
ACCESSION V004404
VERSION V004404
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 5650)
AUTHORS Olson DG, Lynd LR.
TITLE Computational Design and Characterization of a Temperature-sensitive
Replicon for Gram Positive Thermophiles
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 5650)
AUTHORS Olson DG, Lynd LR.
TITLE Direct Submission
JOURNAL Submitted (23-FEB-2011) Thayer School of Engineering, Dartmouth
College, 8000 Cummings, Hanover, NH 03755, USA
REFERENCE 3 (bases 1 to 5650)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 5650)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(23-FEB-2011) Thayer School of Engineering, Dartmouth College, 8000
Cummings, Hanover, NH 03755, USA"
SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..5650
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin 1..589
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS 1281..2282
/label="repB"
/note="RepB replication protein"
promoter 2400..2504
/label="AmpR promoter"
CDS 2505..3362
/label="AmpR"
/note="beta-lactamase"
protein_bind 3524..3540
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 3548..3564
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
terminator 3656..3683
/label="T7Te terminator"
/note="phage T7 early transcription terminator"
CDS complement(3767..4414)
/gene="cat"
/label="Chloramphenicol acetyltransferase"
/note="Chloramphenicol acetyltransferase from
Staphylococcus aureus. Accession#: P00485"
regulatory complement(4415..4942)
/gene="gapDH"
/label="derived from Clostridium thermocellum DSM1313"
/note="derived from Clostridium thermocellum DSM1313"
/regulatory_class="promoter"
gene complement(4415..4942)
/gene="gapDH"
/label="gapDH"
/note="derived from Clostridium thermocellum DSM1313"
terminator complement(5049..5076)
/label="rrnB T2 terminator"
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator complement(5168..5249)
/label="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
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