pMU770 vector (V004404)

LOCUS       V004404                 5650 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004404
VERSION     V004404
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5650)
  AUTHORS   Olson DG, Lynd LR.
  TITLE     Computational Design and Characterization of a Temperature-sensitive
            Replicon for Gram Positive Thermophiles
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5650)
  AUTHORS   Olson DG, Lynd LR.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-FEB-2011) Thayer School of Engineering, Dartmouth
            College, 8000 Cummings, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 5650)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5650)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (23-FEB-2011) Thayer School of Engineering, Dartmouth College, 8000
            Cummings, Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5650
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             1281..2282
                     /label="repB"
                     /note="RepB replication protein"
     promoter        2400..2504
                     /label="AmpR promoter"
     CDS             2505..3362
                     /label="AmpR"
                     /note="beta-lactamase"
     protein_bind    3524..3540
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3548..3564
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     terminator      3656..3683
                     /label="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     CDS             complement(3767..4414)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     regulatory      complement(4415..4942)
                     /gene="gapDH"
                     /label="derived from Clostridium thermocellum DSM1313"
                     /note="derived from Clostridium thermocellum DSM1313"
                     /regulatory_class="promoter"
     gene            complement(4415..4942)
                     /gene="gapDH"
                     /label="gapDH"
                     /note="derived from Clostridium thermocellum DSM1313"
     terminator      complement(5049..5076)
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(5168..5249)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"