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pMU102 vector (V004411)

Basic Vector Information

Vector Name:
pMU102
Length:
3568 bp
Type:
Expression vector
Replication origin:
ori
Source/Author:
Olson DG, Lynd LR.

pMU102 vector Vector Map

pMU1023568 bp6001200180024003000orirepBChloramphenicol acetyltransferaseCAP binding sitelac promoterlac operatorM13 revMCS

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pMU102 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       V004411                 3568 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004411
VERSION     V004411
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Olson DG, Lynd LR.
  TITLE     Computational Design and Characterization of a Temperature-sensitive
            Replicon for Gram Positive Thermophiles
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Olson DG, Lynd LR.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-FEB-2011) Thayer School of Engineering, Dartmouth
            College, 8000 Cummings, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 3568)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3568)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (23-FEB-2011) Thayer School of Engineering, Dartmouth College, 8000
            Cummings, Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3568
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             1281..2282
                     /label="repB"
                     /note="RepB replication protein"
     CDS             2392..3039
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     protein_bind    3172..3193
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3208..3238
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    3246..3262
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3270..3286
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    complement(3299..3355)
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"

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