pMSP3535H3 vector (V004455)

LOCUS       V004455                 9463 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004455
VERSION     V004455
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9463)
  AUTHORS   Oddone GM, Mills DA, Block DE.
  TITLE     Incorporation of nisI-mediated nisin immunity improves vector-based
            nisin-controlled gene expression in lactic acid bacteria
  JOURNAL   Plasmid 61 (3), 151-158 (2009)
   PUBMED   19141301
REFERENCE   2  (bases 1 to 9463)
  AUTHORS   Oddone GM, Kim J-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-DEC-2008) Viticulture and Enology, University of
            California, Davis, RMI North Lab, 595 Hilgard Lane, Davis, CA 95616,
            USA
REFERENCE   3  (bases 1 to 9463)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9463)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid";
            date: "2009"; volume: "61"; issue: "3"; pages: "151-158"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (16-DEC-2008) Viticulture and Enology, University of California,
            Davis, RMI North Lab, 595 Hilgard Lane, Davis, CA 95616, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9463
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             332..1066
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     CDS             1971..2270
                     /codon_start=1
                     /gene="repD"
                     /product="RepD"
                     /label="repD"
                     /note="replication factor"
                     /protein_id="ACM62692.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKSD
                     KYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            1971..2270
                     /gene="repD"
                     /label="repD"
     CDS             2313..3803
                     /codon_start=1
                     /gene="repE"
                     /product="RepE"
                     /label="repE"
                     /note="replication factor"
                     /protein_id="ACM62693.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRSK
                     SSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQINTF
                     FIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSEFKS
                     VKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQDWSFK
                     QTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTLSLAYF
                     SSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILCKAWVSS
                     DLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVTTKKEIRE
                     VLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKKEEKESYIK
                     ALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            2313..3803
                     /gene="repE"
                     /label="repE"
     CDS             4153..4290
                     /codon_start=1
                     /gene="repG"
                     /product="RepG"
                     /label="repG"
                     /note="replication factor"
                     /protein_id="ACM62694.1"
                     /translation="MEKYLKNPITWIGLVLVVTWFLTKSSEFLIFGVCVLLLVFASQSD
                     "
     gene            4153..4290
                     /gene="repG"
                     /label="repG"
     CDS             4440..5177
                     /codon_start=1
                     /gene="nisI"
                     /product="NisI"
                     /label="nisI"
                     /note="nisin immunity protein; provides significant
                     protection from the toxic effects of the inducer nisin"
                     /protein_id="ACM62695.1"
                     /translation="MRKYLILIVALIGITGLSGCYQTSQKKVRFDEGSYTNFIYDNKSY
                     FVTDKEIPQENVNNCKVKFYNLLIVDMKSEKLLSSSNKNSVTLVLNNIYEASDKSLCMG
                     INDRYYKILPESDKGVVKALRLQNFDVTSDISDDNFVIDKNDSRKIDYMGNIYSISDTT
                     VSDEELGEYQDVLAEVRVFDSVSGKSIPRSEWGRIDKDGSNSKQSRTEWDYGEIHSIRG
                     KSLTEAFAVEINDDFKLATKVGN"
     gene            4440..5177
                     /gene="nisI"
                     /label="nisI"
     CDS             5406..6044
                     /codon_start=1
                     /gene="nisR"
                     /product="NisR"
                     /label="nisR"
                     /note="nis operon regulator protein"
                     /protein_id="ACM62696.1"
                     /translation="MKTALEMRNYEVATHQNISLPLDITDFQGFDLILLDIMMSNIEGT
                     EICKRIRREISTPIIFVSAKDTEEDIINGLGIGGDDYITKPFSLKQLVAKVEANIKREE
                     RNKHAVHVFSEIRRDLGPITFYLEERRVCVNGQTIPLTCREYDILELLSQRTSKVYTRE
                     DIYDDVYDEYSNALFRSISEYIYQIRSKFAPYDINPIKTVRGLGYQWHG"
     gene            5406..6044
                     /gene="nisR"
                     /label="nisR"
     CDS             6037..7377
                     /gene="nisK"
                     /label="Nisin biosynthesis sensor protein NisK"
                     /note="Nisin biosynthesis sensor protein NisK from
                     Lactococcus lactis subsp. lactis. Accession#: P42707"
     rep_origin      7882..8470
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        8728..8746
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     9147..9163
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    9164..9220
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(9233..9249)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"