pMSP3535-RT vector (V004457)

LOCUS       V004457                 8588 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004457
VERSION     V004457
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8588)
  AUTHORS   Staddon JH, Bryan EM, Manias DA, Dunny GM.
  TITLE     Conserved target for group II intron insertion in relaxase genes of
            conjugative elements of gram-positive bacteria
  JOURNAL   J. Bacteriol. 186 (8), 2393-2401 (2004)
   PUBMED   15060042
REFERENCE   2  (bases 1 to 8588)
  AUTHORS   Staddon JH, Bryan EM, Manias DA, Dunny GM.
  TITLE     Nisin-inducible pCF10 target of Ll.ltrB
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 8588)
  AUTHORS   Staddon JH, Bryan EM, Manias DA, Dunny GM.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAY-2003) Microbiology, University of Minnesota, MMC
            196, 420 Delaware Street S.E., Minneapolis, MN 55455, USA
REFERENCE   4  (bases 1 to 8588)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 8588)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Bacteriol."; date: "2004"; volume: "186"; issue: "8"; pages:
            "2393-2401"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (21-MAY-2003) Microbiology, University of Minnesota, MMC 196, 420
            Delaware Street S.E., Minneapolis, MN 55455, USA"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8588
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             332..1066
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     CDS             2046..2345
                     /codon_start=1
                     /product="RepD"
                     /label="RepD"
                     /protein_id="AAQ75108.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKSD
                     KYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     CDS             2388..3878
                     /codon_start=1
                     /product="RepE"
                     /label="RepE"
                     /note="plasmid replication; pAMbeta1 plasmid replicon"
                     /protein_id="AAQ75109.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRSK
                     SSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQINTF
                     FIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSEFKS
                     VKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQDWSFK
                     QTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTLSLAYF
                     SSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILCKAWVSS
                     DLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVTTKKEIRE
                     VLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKKEEKESYIK
                     ALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     CDS             4228..4365
                     /codon_start=1
                     /product="RepG"
                     /label="RepG"
                     /protein_id="AAQ75110.1"
                     /translation="MEKYLKNPITWIGLVLVVTWFLTKSSEFLIFGVCVLLLVFASQSD
                     "
     CDS             4614..5252
                     /codon_start=1
                     /product="NisR"
                     /label="NisR"
                     /note="response regulator"
                     /protein_id="AAQ75111.1"
                     /translation="MKTALEMRNYEVATHQNISLPLDITDFQGFDLILLDIMMSNIEGT
                     EICKRIRREISTPIIFVSAKDTEEDIINGLGIGGDDYITKPFSLKQLVAKVEANIKREE
                     RNKHAVHVFSEIRRDLGPITFYLEERRVCVNGQTIPLTCREYDILELLSQRTSKVYTRE
                     DIYDDVYDEYSNALFRSISEYIYQIRSKFAPYDINPIKTVRGLGYQWHG"
     CDS             5245..6585
                     /gene="nisK"
                     /label="Nisin biosynthesis sensor protein NisK"
                     /note="Nisin biosynthesis sensor protein NisK from
                     Lactococcus lactis subsp. lactis. Accession#: P42707"
     rep_origin      7090..7678
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        7936..7954
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    7976..8246
                     /note="contains nucleotides 308-578 from pcfG gene of
                     pCF10; Ll.ltrB group II intron target; transcribed from
                     PnisA in reverse orientation"
     regulatory      complement(8280..8588)
                     /note="PnisA; nisin-inducible promoter"
                     /regulatory_class="promoter"