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pMSD2 vector (V004461)

Basic Vector Information

Vector Name:
pMSD2
Length:
5833 bp
Type:
Cloning vector
Replication origin:
pBBR1 oriV
Source/Author:
Meng J, Wang H, Liu X, Lin J, Pang X, Lin J.
Promoter:
tac

pMSD2 vector Vector Map

pMSD25833 bp60012001800240030003600420048005400pBBR1 oriVpBBR1 RepM13 fwdT7 promoterMCSlac operatortac promoterstrAstrB

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pMSD2 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_32390        5833 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pMSD2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5833)
  AUTHORS   Meng J, Wang H, Liu X, Lin J, Pang X, Lin J.
  TITLE     Construction of small plasmid vectors for use in genetic improvement
            of the extremely acidophilic Acidithiobacillus caldus
  JOURNAL   Microbiol. Res. 168 (8), 469-476 (2013)
  PUBMED    23639949
REFERENCE   2  (bases 1 to 5833)
  AUTHORS   Meng J, Wang H, Liu X.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-FEB-2013) State Key Laboratory of Microbial 
            Technology, Shandong University, Shanda Nanlu 27#, Jinan, Shandong 
            250100, China
REFERENCE   3  (bases 1 to 5833)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5833)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microbiol. 
            Res."; date: "2013"; volume: "168"; issue: "8"; pages: "469-476"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-FEB-2013) State Key Laboratory of Microbial Technology, Shandong
            University, Shanda Nanlu 27#, Jinan, Shandong 250100, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5833
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1023..1792
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             1793..2452
                     /codon_start=1
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     primer_bind     3162..3178
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3188..3206
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    3215..3322
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     protein_bind    complement(3339..3355)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3363..3391)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     CDS             3656..4459
                     /codon_start=1
                     /gene="strA"
                     /product="Sm resistance protein A"
                     /label=strA
                     /protein_id="AGT62536.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASRR
                     GELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPS
                     MGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPLHDLLARV
                     ERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIA
                     NAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            3656..4459
                     /gene="strA"
                     /label=strA
     CDS             4459..5295
                     /codon_start=1
                     /gene="strB"
                     /product="Sm resistance protein B"
                     /label=strB
                     /protein_id="AGT62537.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
     gene            4459..5295
                     /gene="strB"
                     /label=strB

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