Basic Vector Information
- Vector Name:
- pMRE106_PnptII_eBFP2
- Antibiotic Resistance:
- Ampicillin
- Length:
- 13617 bp
- Type:
- Tn7 delivery vector
- Replication origin:
- pSC101 ori
- Source/Author:
- Remus-Emsermann MNP., Gisler P, Drissner D.
- Promoter:
- araBAD
pMRE106_PnptII_eBFP2 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMRE106_PnptII_eBFP2 vector Sequence
LOCUS 40924_32235 13617 bp DNA circular SYN 18-DEC-2018
DEFINITION Tn7 delivery vector pMRE106_PnptII_eBFP2, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13617)
AUTHORS Remus-Emsermann MNP., Gisler P, Drissner D.
TITLE MiniTn7-transposon delivery vectors for inducible or constitutive
fluorescent protein expression in Enterobacteriaceae
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 13617)
AUTHORS Remus-Emsermann MNP., Drissner D.
TITLE Direct Submission
JOURNAL Submitted (23-MAR-2016) Institute for Food Sciences, Agroscope,
Schloss 1, Waedenswil, Zurich 8820, Switzerland
REFERENCE 3 (bases 1 to 13617)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 13617)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(23-MAR-2016) Institute for Food Sciences, Agroscope, Schloss 1,
Waedenswil, Zurich 8820, Switzerland"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..13617
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS complement(149..1024)
/label=araC
/note="L-arabinose regulatory protein"
promoter 1051..1335
/label=araBAD promoter
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction (Guzman et al., 1995)"
CDS 1453..2271
/gene="tnsA"
/label=tnsA
/note="Transposon Tn7 transposition protein TnsA from
Escherichia coli. Accession#: P13988"
CDS 2261..4366
/gene="tnsB"
/label=tnsB
/note="Transposon Tn7 transposition protein TnsB from
Escherichia coli. Accession#: P13989"
CDS 4366..6030
/gene="tnsC"
/label=tnsC
/note="Transposon Tn7 transposition protein TnsC from
Escherichia coli. Accession#: P05846"
CDS 6036..7562
/codon_start=1
/gene="tnsD"
/product="TnsD"
/label=tnsD
/note="Tn7 transposition site-selector"
/protein_id="AMZ00016.1"
/translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVAT
LGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQGAV
HLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVFFDR
AVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQWTLFY
QRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFRKHRKA
FSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVKHKDWQQ
LVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDWNQRDRIA
VRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKRYSESVEDY
QIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE"
gene 6036..7562
/gene="tnsD"
/label=tnsD
terminator 8196..8282
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 8374..8401
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
promoter 8420..8511
/label=AmpR promoter
CDS 8512..9369
/label=AmpR
/note="beta-lactamase"
oriT complement(9543..9652)
/direction=LEFT
/label=oriT
/note="incP origin of transfer"
rep_origin 10392..10614
/label=pSC101 ori
/note="low-copy replication origin that requires the Rep101
protein"
CDS 10662..11609
/label=Rep101(Ts)
/note="temperature-sensitive version of the RepA protein
needed for replication with the pSC101 origin (Armstrong et
al., 1984)"
mobile_element 12045..12210
/label=Tn7L
/note="mini-Tn7 element (left end of the Tn7 transposon)"
regulatory 12294..12612
/label=nptII promoter
/note="nptII promoter"
/regulatory_class="promoter"
CDS 12642..13358
/label=EBFP2
/note="enhanced blue variant of GFP (Ai et al., 2007)"
repeat_region 13406..13617
/label=Tn7 right end
/note="Tn7 right end"
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