pMR361-K vector (V004482)

LOCUS       40924_32195        7836 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pMR361-K, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7836)
  AUTHORS   Narayanan AM, Ramsey MM, Stacy A, Whiteley M.
  TITLE     Genomic resources for an oral pathogen
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7836)
  AUTHORS   Ramsey MM.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2017) Department of Molecular Biosciences, The 
            University of Texas at Austin, 2506 Speedway, NMS 3.254, Austin, TX 
            78712, USA
REFERENCE   3  (bases 1 to 7836)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7836)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-MAR-2017) Department of Molecular Biosciences, The University of
            Texas at Austin, 2506 Speedway, NMS 3.254, Austin, TX 78712, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7836
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            35..144
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             177..545
                     /label=traJ
                     /note="oriT-recognizing protein"
     CDS             604..1344
                     /codon_start=1
                     /product="TraI"
                     /label=TraI
                     /protein_id="ARI46004.1"
                     /translation="MRSIKKSDFAELVKYITDEQGKTERLGHVRVTNCEANTLPAVMAE
                     VMATQHGNTRSEADKTYHLLVSFRAGEKPDAETLRAIEDRICAGLGFAEHQRVSAVHHD
                     TDNLHIHIAINKIHPTRNTIHEPYRAYRALADLCATLERDYGLERDNHETRQRVSENRA
                     NDMERHAGVESLVGWILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFG
                     VGMVAGPVAGGLLGAISLLPRAFR"
     promoter        complement(1616..1720)
                     /label=AmpR promoter
     rep_origin      1918..2506
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2794..2815
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2830..2860
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2868..2884
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2892..2908
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2962..4008
                     /codon_start=1
                     /product="transposase"
                     /label=transposase
                     /protein_id="ARI46007.1"
                     /translation="MEKKEFRVLIKYCFLKGKNTVEAKTWLDNEFPDSAPGKSTIIDWY
                     AKFKRGEMSTEDGERSGRPKEVVTDENIKKIHKMILNDRKMKLIEIAEALKISKERVGH
                     IIHQYLDMRKLCAKWVPRELTFDQKQRRVDDSKRCLQLLTRNTPEFFRRYVTMDETWLH
                     HYTPESNRQSAEWTATGEPSPKRGKTQKSAGKVMASVFWDAHGIIFIDYLEKGKTINSD
                     YYMALLERLKVEIAAKRPHMKKKKVLFHQDNAPCHKSLRTMAKIHELGFELLPHPPYSP
                     DLAPSDFFLFSDLKRMLAGKKFGCNEEVIAETEAYFEAKPKEYYQNGIKKLEGRYNRCI
                     ALEGNYVE"
     mobile_element  4020..4098
                     /mobile_element_type="transposon:mariner"
                     /label=ner
                     /note="repeat end"
     promoter        complement(4104..4122)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     4123..4139
                     /note="M13 rev; common sequencing primer, one of multiple 
                     similar variants"
     CDS             complement(4208..4999)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        5517..5535
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     mobile_element  5541..5640
                     /mobile_element_type="transposon:mariner"
                     /label=ner
                     /note="repeat end"
     CDS             6012..6686
                     /codon_start=1
                     /product="spectinomycin resistance cassette"
                     /label=spectinomycin resistance cassette
                     /note="SpcR"
                     /protein_id="ARI46006.1"
                     /translation="MFGSGVESGLKPNSDLDFLVVVSEPLTDQSKEILIQKIRPISKKI
                     GDKSNLRYIELTIIIQQEMVPWNHPPKQEFIYGEWLQELYEQGYIPQKELNSDLTIMLY
                     QAKRKNKRIYGNYDLEELLPDIPFSDVRRAIMDSSEELIDNYQDDETNSILTLCRMILT
                     MDTGKIIPKDIAGNAVAESSPLEHRERILLAVRSYLGENIEWTNENVNLTINYLNNRLK
                     KL"
     rep_origin      complement(6871..7227)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"