Basic Vector Information
- Vector Name:
- pMQ95
- Antibiotic Resistance:
- Ampicillin
- Length:
- 8905 bp
- Type:
- Shuttle vector
- Replication origin:
- oriV
- Source/Author:
- Shanks RM, Caiazza NC, Hinsa SM, Toutain CM, O'Toole GA.
- Promoter:
- araBAD
pMQ95 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMQ95 vector Sequence
LOCUS 40924_32185 8905 bp DNA circular SYN 18-DEC-2018
DEFINITION Shuttle vector pMQ95, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8905)
AUTHORS Shanks RM, Caiazza NC, Hinsa SM, Toutain CM, O'Toole GA.
TITLE Saccharomyces cerevisiae-based molecular tool kit for manipulation
of genes from gram-negative bacteria
JOURNAL Appl. Environ. Microbiol. 72 (7), 5027-5036 (2006)
PUBMED 16820502
REFERENCE 2 (bases 1 to 8905)
AUTHORS Shanks RM, Caiazza NC, Hinsa SM, Toutain CM, O'Toole GA.
TITLE Direct Submission
JOURNAL Submitted (18-MAY-2006) Microbiology and Immunology, Dartmouth
Medical School, Vail Building Room 505, Hanover, NH 03755, USA
REFERENCE 3 (bases 1 to 8905)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 8905)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
Environ. Microbiol."; date: "2006"; volume: "72"; issue: "7"; pages:
"5027-5036"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(18-MAY-2006) Microbiology and Immunology, Dartmouth Medical School,
Vail Building Room 505, Hanover, NH 03755, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..8905
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 1..801
/label=URA3
/note="orotidine-5'-phosphate decarboxylase, required for
uracil biosynthesis"
oriT complement(893..1001)
/direction=LEFT
/label=oriT
/note="incP origin of transfer"
rep_origin 1198..1728
/label=oriV
/note="incP origin of replication"
CDS 2120..3265
/label=trfA
/note="trans-acting replication protein that binds to and
activates oriV"
terminator complement(3525..3611)
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
CDS complement(3841..4554)
/label=yeGFP
/note="yeast-enhanced green fluorescent protein"
regulatory complement(4562..4567)
/label=artificial RBS
/note="artificial RBS"
/regulatory_class="ribosome_binding_site"
promoter complement(4612..4896)
/label=araBAD promoter
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction (Guzman et al., 1995)"
CDS 4923..5798
/label=araC
/note="L-arabinose regulatory protein"
rep_origin complement(6156..6744)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(6918..7775)
/label=AmpR
/note="beta-lactamase"
promoter complement(7776..7880)
/label=AmpR promoter
misc_feature 7917..8420
/label=CEN/ARS
/note="S. cerevisiae CEN6 centromere fused to an
autonomously replicating sequence"
promoter 8685..8905
/label=URA3 promoter
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