Basic Vector Information
- Vector Name:
- pMQ354
- Length:
- 8422 bp
- Type:
- Promoter probe vector
- Replication origin:
- R6K γ ori
- Host:
- Yeast
- Source/Author:
- Kalivoda EJ, Horzempa J, Stella NA, Sadaf A, Kowalski RP, Nau GJ, Shanks RM.
- Promoter:
- URA3
pMQ354 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMQ354 vector Sequence
LOCUS 40924_32100 8422 bp DNA circular SYN 18-DEC-2018 DEFINITION Promoter probe vector pMQ354, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8422) AUTHORS Kalivoda EJ, Horzempa J, Stella NA, Sadaf A, Kowalski RP, Nau GJ, Shanks RM. TITLE New vector tools with a hygromycin resistance marker for use with opportunistic pathogens JOURNAL Mol. Biotechnol. 48 (1), 7-14 (2011) PUBMED 20972648 REFERENCE 2 (bases 1 to 8422) AUTHORS Kalivoda EJ, Horzempa JE, Sadaf AA, Stella NA, Nau GJ, Shanks RM. TITLE Direct Submission JOURNAL Submitted (19-DEC-2009) Ophthalmology, University of Pittsburgh, EEI 1015, 203 Lothrop St, Pittsburgh, PA 15213, USA REFERENCE 3 (bases 1 to 8422) TITLE Direct Submission REFERENCE 4 (bases 1 to 8422) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Biotechnol."; date: "2011"; volume: "48"; issue: "1"; pages: "7-14" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (19-DEC-2009) Ophthalmology, University of Pittsburgh, EEI 1015, 203 Lothrop St, Pittsburgh, PA 15213, USA" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..8422 /mol_type="other DNA" /organism="synthetic DNA construct" oriT complement(352..461) /direction=LEFT /label=oriT /note="incP origin of transfer" terminator complement(882..909) /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator complement(1001..1087) /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" CDS complement(1170..1187) /label=6xHis /note="6xHis affinity tag" CDS complement(1197..1238) /label=V5 tag /note="epitope tag from simian virus 5" CDS complement(1269..4313) /label=lacZ /note="beta-galactosidase" protein_bind complement(4361..4377) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4385..4415) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(4430..4451) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter complement(4502..4520) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" rep_origin 4728..5116 /label=R6K gamma ori /note="gamma replication origin from E. coli plasmid R6K; requires the R6K initiator protein pi for replication" CDS 5559..6554 /gene="hyg" /label=hyg /note="Hygromycin-B 7''-O-kinase from Streptomyces hygroscopicus. Accession#: P09979" CDS complement(6823..7623) /label=URA3 /note="orotidine-5'-phosphate decarboxylase, required for uracil biosynthesis" promoter complement(7624..7844) /label=URA3 promoter misc_feature 7872..8375 /label=CEN/ARS /note="S. cerevisiae CEN6 centromere fused to an autonomously replicating sequence"
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