Basic Vector Information
- Vector Name:
- pMQ351
- Length:
- 9921 bp
- Type:
- Cloning vector
- Replication origin:
- R6K γ ori
- Host:
- Yeast
- Source/Author:
- Kalivoda EJ, Horzempa J, Stella NA, Sadaf A, Kowalski RP, Nau GJ, Shanks RM.
- Promoter:
- LEU2
pMQ351 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMQ351 vector Sequence
LOCUS 40924_32085 9921 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pMQ351, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9921)
AUTHORS Kalivoda EJ, Horzempa J, Stella NA, Sadaf A, Kowalski RP, Nau GJ,
Shanks RM.
TITLE New vector tools with a hygromycin resistance marker for use with
opportunistic pathogens
JOURNAL Mol. Biotechnol. 48 (1), 7-14 (2011)
PUBMED 20972648
REFERENCE 2 (bases 1 to 9921)
AUTHORS Kalivoda EJ, Horzempa J, Sadaf A, Stella NA, Nau GJ, Shanks RM.
TITLE Direct Submission
JOURNAL Submitted (18-OCT-2010) Ophthalmology, University of Pittsburgh, 203
Lothrop St, Pittsburgh, PA 15213, USA
REFERENCE 3 (bases 1 to 9921)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 9921)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Mol.
Biotechnol."; date: "2011"; volume: "48"; issue: "1"; pages: "7-14"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(18-OCT-2010) Ophthalmology, University of Pittsburgh, 203 Lothrop
St, Pittsburgh, PA 15213, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..9921
/mol_type="other DNA"
/organism="synthetic DNA construct"
oriT complement(399..508)
/direction=LEFT
/label=oriT
/note="incP origin of transfer"
terminator complement(929..956)
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator complement(1048..1134)
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
primer_bind 1484..1500
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
misc_feature complement(1504..1560)
/label=MCS
/note="pUC18/19 multiple cloning site"
primer_bind complement(1570..1586)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 1594..1610
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(1618..1648)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(1663..1684)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin 1969..2357
/label=R6K gamma ori
/note="gamma replication origin from E. coli plasmid R6K;
requires the R6K initiator protein pi for replication"
CDS complement(2892..3593)
/label=dTomato
/note="dimeric variant of DsRed fluorescent protein (Shaner
et al., 2004)"
CDS 5380..5394
/label=enterokinase site
/note="enterokinase recognition and cleavage site"
CDS complement(5635..6327)
/gene="SCEI"
/label=SCEI
/note="Intron-encoded endonuclease I-SceI from
Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
Accession#: P03882"
CDS complement(6355..7350)
/gene="hyg"
/label=hyg
/note="Hygromycin-B 7''-O-kinase from Streptomyces
hygroscopicus. Accession#: P09979"
CDS complement(7639..8730)
/label=LEU2
/note="3-isopropylmalate dehydrogenase, required for
leucine biosynthesis"
promoter complement(8743..9147)
/label=LEU2 promoter
misc_feature 9418..9921
/label=CEN/ARS
/note="S. cerevisiae CEN6 centromere fused to an
autonomously replicating sequence"
This page is informational only.