Basic Vector Information
pSC101 ori is a low-copy replication origin that requires the Rep101 protein, which is a temperature-sensitive protein. When bacteria containing a plasmid with this ori is cultured at 37℃, the plasmid will be lost.
- Vector Name:
- pMQ299
- Length:
- 6913 bp
- Type:
- Delivery vector
- Replication origin:
- pSC101 ori
- Host:
- Yeast
- Source/Author:
- Kalivoda EJ, Horzempa J, Stella NA, Sadaf A, Kowalski RP, Nau GJ, Shanks RM.
- Copy Number:
- Low Copy
- Promoter:
- URA3
- Growth Temperature:
- 30℃
pMQ299 vector Map
pMQ299 vector Sequence
LOCUS V004509 6913 bp DNA circular SYN 18-DEC-2018
DEFINITION Exported.
ACCESSION V004509
VERSION V004509
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 6913)
AUTHORS Kalivoda EJ, Horzempa J, Stella NA, Sadaf A, Kowalski RP, Nau GJ,
Shanks RM.
TITLE New vector tools with a hygromycin resistance marker for use with
opportunistic pathogens
JOURNAL Mol. Biotechnol. 48 (1), 7-14 (2011)
PUBMED 20972648
REFERENCE 2 (bases 1 to 6913)
AUTHORS Kalivoda EJ, Horzempa JE, Sadaf AA, Stella NA, Nau GJ, Shanks RM.
TITLE Direct Submission
JOURNAL Submitted (19-DEC-2009) Ophthalmology, University of Pittsburgh, EEI
1015, 203 Lothrop St, Pittsburgh, PA 15213, USA
REFERENCE 3 (bases 1 to 6913)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 6913)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Mol.
Biotechnol."; date: "2011"; volume: "48"; issue: "1"; pages: "7-14"
SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(19-DEC-2009) Ophthalmology, University of Pittsburgh, EEI 1015, 203
Lothrop St, Pittsburgh, PA 15213, USA"
SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..6913
/mol_type="other DNA"
/organism="synthetic DNA construct"
oriT complement(399..508)
/direction=LEFT
/label="oriT"
/note="incP origin of transfer"
terminator complement(929..956)
/label="rrnB T2 terminator"
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator complement(1048..1134)
/label="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
primer_bind 1484..1500
/label="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
CDS complement(1524..2216)
/gene="SCEI"
/label="Intron-encoded endonuclease I-SceI"
/note="Intron-encoded endonuclease I-SceI from
Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
Accession#: P03882"
protein_bind complement(2246..2262)
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(2270..2300)
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind complement(2315..2336)
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin 2523..2745
/label="pSC101 ori"
/note="low-copy replication origin that requires the Rep101
protein"
CDS 2793..3740
/label="Rep101(Ts)"
/note="temperature-sensitive version of the RepA protein
needed for replication with the pSC101 origin (Armstrong et
al., 1984)"
CDS complement(4031..5026)
/gene="hyg"
/label="Hygromycin-B 7''-O-kinase"
/note="Hygromycin-B 7''-O-kinase from Streptomyces
hygroscopicus. Accession#: P09979"
promoter complement(5157..5261)
/label="AmpR promoter"
CDS complement(5361..6161)
/label="URA3"
/note="orotidine-5'-phosphate decarboxylase, required for
uracil biosynthesis"
promoter complement(6162..6382)
/label="URA3 promoter"
misc_feature 6410..6913
/label="CEN/ARS"
/note="S. cerevisiae CEN6 centromere fused to an
autonomously replicating sequence"
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