pRRL-sffv-eGFP-cmv-hsCDNF vector (V003621)

LOCUS       V003621                 8624 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003621
VERSION     V003621
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8624)
  AUTHORS   Cordero Llana O.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-APR-2014) Physiology Anatomy and Genetics, University
            of Oxford, South Parks Rd., Oxford, England OX1 3QX, United Kingdom
REFERENCE   2  (bases 1 to 8624)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 8624)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (12-APR-2014) Physiology Anatomy and Genetics, University of Oxford,
            South Parks Rd., Oxford, England OX1 3QX, United Kingdom"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8624
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    52..640
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     misc_feature    710..725
                     /label="cPPPT5'"
                     /note="cPPPT5'"
     LTR             727..960
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1032..1166
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      1172..1307
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(1340..1358)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1368..1384)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      1529..1984
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2013..2117
                     /label="AmpR promoter"
     CDS             2118..2975
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3149..3737
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    4025..4046
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4061..4091
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    4099..4115
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4123..4139
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        4160..4178
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4206..4433
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     misc_feature    4434..4614
                     /label="HIV-LTR"
                     /note="HIV-LTR"
     LTR             4434..4614
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4658..4783
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    5276..5509
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             5694..5738
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             5887..5928
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    5999..6116
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        6233..6640
                     /label="SFFV promoter"
                     /note="spleen focus-forming virus long terminal repeat
                     (LTR) promoter"
     CDS             6662..7378
                     /label="EGFP"
                     /note="enhanced GFP"
     enhancer        7458..7761
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7762..7965
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             8060..8620
                     /gene="CDNF"
                     /label="Cerebral dopamine neurotrophic factor"
                     /note="Cerebral dopamine neurotrophic factor from Homo
                     sapiens. Accession#: Q49AH0"