Basic Vector Information
prpL23 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
prpL23 vector Sequence
LOCUS 40924_37613 3914 bp DNA circular SYN 18-DEC-2018 DEFINITION Expression vector prpL23, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3914) AUTHORS Sorensen HP, Kristensen JE, Sperling-Petersen HU, Mortensen KK. TITLE Soluble expression of aggregating proteins by covalent coupling to the ribosome JOURNAL Unpublished REFERENCE 2 (bases 1 to 3914) AUTHORS Sorensen HP, Sperling-Petersen HU, Mortensen KK. TITLE Direct Submission JOURNAL Submitted (29-MAR-2004) Department of Molecular Biology, Aarhus University, Gustav Wieds Vej 10C, Aarhus C 8000, Denmark REFERENCE 3 (bases 1 to 3914) TITLE Direct Submission REFERENCE 4 (bases 1 to 3914) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (29-MAR-2004) Department of Molecular Biology, Aarhus University, Gustav Wieds Vej 10C, Aarhus C 8000, Denmark" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..3914 /mol_type="other DNA" /organism="synthetic DNA construct" CDS complement(78..734) /label=CmR /note="chloramphenicol acetyltransferase" promoter complement(735..837) /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" rep_origin complement(1363..1907) /direction=LEFT /label=p15A ori /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin." CDS complement(2049..2924) /label=araC /note="L-arabinose regulatory protein" promoter 2951..3235 /label=araBAD promoter /note="promoter of the L-arabinose operon of E. coli; the araC regulatory gene is transcribed in the opposite direction (Guzman et al., 1995)" RBS 3262..3284 /label=RBS /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" CDS 3292..3591 /gene="rplW" /label=rplW /note="Large ribosomal subunit protein uL23 from Escherichia coli (strain K12). Accession#: P0ADZ0" CDS 3592..3609 /label=6xHis /note="6xHis affinity tag" terminator 3840..3886 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene"
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