prpL23 vector (V003638)

Basic Vector Information

      • Vector Name:
      • prpL23
      • Antibiotic Resistance:
      • Chloramphenicol
      • Length:
      • 3914 bp
      • Type:
      • Expression vector
      • Replication origin:
      • p15A ori
      • Source/Author:
      • Sorensen HP, Kristensen JE, Sperling-Petersen HU, Mortensen KK.
      • Promoter:
      • araBAD

prpL23 vector Vector Map

prpL233914 bp60012001800240030003600CmRcat promoterp15A oriaraCaraBAD promoterRBSrplW6xHisrrnB T1 terminator

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

prpL23 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_37613        3914 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector prpL23, complete sequence.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Sorensen HP, Kristensen JE, Sperling-Petersen HU, Mortensen KK.
  TITLE     Soluble expression of aggregating proteins by covalent coupling to 
            the ribosome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Sorensen HP, Sperling-Petersen HU, Mortensen KK.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2004) Department of Molecular Biology, Aarhus 
            University, Gustav Wieds Vej 10C, Aarhus C 8000, Denmark
REFERENCE   3  (bases 1 to 3914)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3914)
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-MAR-2004) Department of Molecular Biology, Aarhus University, 
            Gustav Wieds Vej 10C, Aarhus C 8000, Denmark"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3914
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(78..734)
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(735..837)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      complement(1363..1907)
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(2049..2924)
                     /note="L-arabinose regulatory protein"
     promoter        2951..3235
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     RBS             3262..3284
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3292..3591
                     /note="Large ribosomal subunit protein uL23 from
                     Escherichia coli (strain K12). Accession#: P0ADZ0"
     CDS             3592..3609
                     /note="6xHis affinity tag"
     terminator      3840..3886
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB

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