pRP-M-GFP-C vector (V003650)

LOCUS       40924_37553        8426 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pRP-M-GFP-C, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8426)
  AUTHORS   Soysa R, Tran KD, Ullman B, Yates PA.
  TITLE     Integrating ribosomal promoter vectors that offer a choice of 
            constitutive expression profiles in Leishmania donovani
  JOURNAL   Mol. Biochem. Parasitol. (2016) In press
  PUBMED    26844641
REFERENCE   2  (bases 1 to 8426)
  AUTHORS   Yates PA, Ullman B, Soysa R.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 8426)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8426)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. 
            Biochem. Parasitol. (2016) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-DEC-2015) Biochemistry "
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8426
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             981..1781
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
     polyA_signal    1791..1839
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(1962..1973)
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     rep_origin      complement(3462..4050)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4224..5081)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5082..5186)
                     /label=AmpR promoter
     misc_feature    5356..6003
                     /note="derived from Leishmania donovani ribosomal RNA
                     spacer region"
     regulatory      6030..6579
                     /note="derived from Leishmania donovani ribosomal RNA
                     genes"
                     /regulatory_class="promoter"
     misc_feature    6601..6682
                     /note="contains artificial trans-splice signal and 5-UTR; 
                     leishmanial consensus splice acceptor site; LdPARP"
     misc_feature    6689..6768
                     /label=multiple cloning site
                     /note="multiple cloning site"
     CDS             6800..7513
                     /label=EGFP
                     /note="enhanced GFP"
     misc_feature    7527..8411
                     /note="derived from Leishmania major alpha tubulin
                     intergenic region; LmTub"