prME-def vector (V003675)

Basic Vector Information

      • Vector Name:
      • prME-def
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 12687 bp
      • Type:
      • YFV replicon vector
      • Replication origin:
      • p15A ori
      • Source/Author:
      • Shustov AV, Frolov IV.

prME-def vector Vector Map

prME-def12687 bp60012001800240030003600420048005400600066007200780084009000960010200108001140012000126005'UTR of Yellow fever virus 17DYellow fever virus 17D capsid proteinfirst 6 aa from pr Yellow fever virus 17DmCherrylast 49 aa from E of Yellow fever virus 17DNS1 of Yellow fever virus 17DNS2 of Yellow fever virus 17DNS3 of Yellow fever virus 17DNS4 of Yellow fever virus 17DNS5 of Yellow fever virus 17D3'UTR of Yellow fever virus 17Dantigenomic ribozyme of HDVp15A oriAmpRAmpR promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

prME-def vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_37403       12687 bp DNA     circular SYN 18-DEC-2018
DEFINITION  YFV replicon vector prME-def, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12687)
  AUTHORS   Shustov AV, Frolov IV.
  TITLE     Trans-packaging of Yellow Fever virus replicons: an approach for 
            flaviviral antigen production
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12687)
  AUTHORS   Shustov AV, Frolov IV.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-DEC-2005) Department of Microbiology and Immunology, 
            University of Texas Medical Branch, 301 University Boulevard, 
            Galveston, TX 77550-1019, USA
REFERENCE   3  (bases 1 to 12687)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12687)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-DEC-2005) Department of Microbiology and Immunology, University 
            of Texas Medical Branch, 301 University Boulevard, Galveston, TX 
            77550-1019, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12687
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          2..506
                     /mol_type="other DNA"
                     /db_xref="taxon:11090"
                     /organism="Yellow fever virus 17D"
     source          1215..9774
                     /mol_type="other DNA"
                     /db_xref="taxon:11090"
                     /organism="Yellow fever virus 17D"
     misc_feature    2..119
                     /label=5'UTR of Yellow fever virus 17D
                     /note="5'UTR of Yellow fever virus 17D"
     misc_feature    120..482
                     /label=Yellow fever virus 17D capsid protein
                     /note="Yellow fever virus 17D capsid protein"
     misc_feature    483..500
                     /label=first 6 aa from pr Yellow fever virus 17D
                     /note="first 6 aa from pr Yellow fever virus 17D"
     CDS             507..1214
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     misc_feature    1218..1364
                     /label=last 49 aa from E of Yellow fever virus 17D
                     /note="last 49 aa from E of Yellow fever virus 17D"
     misc_feature    1365..2591
                     /label=NS1 of Yellow fever virus 17D
                     /note="NS1 of Yellow fever virus 17D"
     misc_feature    2592..3482
                     /label=NS2 of Yellow fever virus 17D
                     /note="NS2 of Yellow fever virus 17D"
     misc_feature    3483..5351
                     /label=NS3 of Yellow fever virus 17D
                     /note="NS3 of Yellow fever virus 17D"
     misc_feature    5352..6548
                     /label=NS4 of Yellow fever virus 17D
                     /note="NS4 of Yellow fever virus 17D"
     misc_feature    6549..9266
                     /label=NS5 of Yellow fever virus 17D
                     /note="NS5 of Yellow fever virus 17D"
     misc_feature    9267..9774
                     /label=3'UTR of Yellow fever virus 17D
                     /note="3'UTR of Yellow fever virus 17D"
     misc_feature    9775..10099
                     /label=antigenomic ribozyme of HDV
                     /note="antigenomic ribozyme of HDV"
     polyA_signal    9984..10065
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(10729..11273)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(11441..12298)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(12299..12403)
                     /label=AmpR promoter

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