pRL838 vector (V003709)

LOCUS       V003709                 8850 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003709
VERSION     V003709
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8850)
  AUTHORS   Kaneko T, Nakamura Y, Wolk CP, Kuritz T, Sasamoto S, Watanabe A,
            Iriguchi M, Ishikawa A, Kawashima K, Kimura T, Kishida Y, Kohara M,
            Matsumoto M, Matsuno A, Muraki A, Nakazaki N, Shimpo S, Sugimoto M,
            Takazawa M, Yamada M, Yasuda M, Tabata S.
  TITLE     Complete genomic sequence of the filamentous nitrogen-fixing
            cyanobacterium Anabaena sp. strain PCC 7120
  JOURNAL   DNA Res. 8 (5), 205-213 (2001)
   PUBMED   11759840
REFERENCE   2  (bases 1 to 8850)
  AUTHORS   Wolk CP.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUL-2001) MSU-DOE Plant Research Laboratory, Michigan
            State University, Wilson Road, E. Lansing, MI 48824-1312, USA
REFERENCE   3  (bases 1 to 8850)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8850)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "DNA Res.";
            date: "2001"; volume: "8"; issue: "5"; pages: "205-213"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (30-JUL-2001) MSU-DOE Plant Research Laboratory, Michigan State
            University, Wilson Road, E. Lansing, MI 48824-1312, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8850
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      817..1036
                     /label="ori2"
                     /note="secondary origin of replication for the bacterial F
                     plasmid; also known as oriS"
     CDS             1127..1879
                     /label="repE"
                     /note="replication initiation protein for the bacterial F
                     plasmid"
     misc_feature    1885..2135
                     /label="incC"
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             2461..3633
                     /label="sopA"
                     /note="partitioning protein for the bacterial F plasmid"
     CDS             3636..4604
                     /label="sopB"
                     /note="partitioning protein for the bacterial F plasmid"
     misc_feature    4680..5153
                     /label="sopC"
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(5530..5898)
                     /label="traJ"
                     /note="oriT-recognizing protein"
     oriT            complement(5931..6040)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     misc_feature    6192..6591
                     /label="cos"
                     /note="lambda cos site; allows packaging into phage lambda
                     particles"
     protein_bind    complement(6602..6635)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        6651..6669
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    6673..6832
                     /label="polylinker (multiple cloning site)"
                     /note="polylinker (multiple cloning site)"
     regulatory      6832..6849
                     /note="SP6 promoter from pBAC108L (GenBank Accession Number
                     U51114)"
                     /regulatory_class="promoter"
     promoter        complement(6832..6849)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     regulatory      6865..6891
                     /note="cat promoter from pBR325 (GenBank Accession Number
                     L08855)"
                     /regulatory_class="promoter"
     CDS             6958..7614
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     CDS             7871..8602
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     terminator      8795..8838
                     /label="bacterial terminator"
                     /note="putative bacterial transcription terminator"
     regulatory      8801..8829
                     /note="rrnC terminator from Lorist6 (GenBank Accession
                     Number X98450)"
                     /regulatory_class="terminator"