pRL1383a vector (V003713)

LOCUS       40924_37213        8958 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pRL1383a, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8958)
  AUTHORS   Wolk CP.
  TITLE     pRL1383a, an RSF1010-derived vector designed for subcloning from BAC
            vector pRL838, and for complementation of mutations in Anabaena sp. 
            strain PCC 7120
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8958)
  AUTHORS   Wolk CP.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUL-2001) MSU-DOE Plant Research Laboratory, Michigan 
            State University, Wilson Road, E. Lansing, MI 48824-1312, USA
REFERENCE   3  (bases 1 to 8958)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8958)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-JUL-2001) MSU-DOE Plant Research Laboratory, Michigan State 
            University, Wilson Road, E. Lansing, MI 48824-1312, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8958
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(155..549)
                     /direction=LEFT
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            891..978
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             2217..3185
                     /label=RSF1010 RepB
                     /note="replication protein B of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
     CDS             3699..4535
                     /label=RSF1010 RepA
                     /note="replication protein A of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
     CDS             4525..5373
                     /label=RSF1010 RepC
                     /note="replication protein C of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
     regulatory      complement(5709..5735)
                     /note="trpA terminator from Lorist6 (GenBank Accession
                     Number X98450)"
                     /regulatory_class="terminator"
     misc_feature    5758..6157
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     protein_bind    complement(6168..6201)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        6217..6235
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    6239..6398
                     /label=polylinker (multiple cloning site)
                     /note="polylinker (multiple cloning site)"
     regulatory      6398..6415
                     /note="SP6 promoter from pBAC108L (GenBank Accession Number
                     U51114)"
                     /regulatory_class="promoter"
     promoter        complement(6398..6415)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             7447..8235
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     terminator      8903..8946
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     regulatory      8909..8937
                     /note="rrnC terminator from Lorist6 (GenBank Accession
                     Number X98450)"
                     /regulatory_class="terminator"