pRIT22 vector (V003731)

LOCUS       V003731                 7062 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003731
VERSION     V003731
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7062)
  AUTHORS   Okamoto S, Niki H.
  TITLE     NBRP cloning vector collection sequence project
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7062)
  AUTHORS   Okamoto S, Niki H.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2017) Contact:Sho Okamoto National Institute of
            Genetics, Microbial Genetics Laboratory; 1111 Yata, Mishima,
            Shizuoka 411-8540, Japan URL
            :https://www.nig.ac.jp/labs/MicroGen/index.html
REFERENCE   3  (bases 1 to 7062)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7062)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (07-APR-2017) Contact:Sho Okamoto National Institute of Genetics,
            Microbial Genetics Laboratory; 1111 Yata, Mishima, Shizuoka
            411-8540, Japan URL :https://www.nig.ac.jp/labs/MicroGen/index.html"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7062
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1260..1907
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             complement(2663..2677)
                     /label="enterokinase site"
                     /note="enterokinase recognition and cleavage site"
     CDS             3773..3943
                     /label="ProtA"
                     /note="IgG-binding unit of Staphylococcus aureus protein A"
     misc_feature    4467..4522
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     promoter        4996..5100
                     /label="AmpR promoter"
     CDS             5101..5958
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6132..6720
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"