pRIT21 vector (V003732)

LOCUS       V003732                 7064 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003732
VERSION     V003732
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7064)
  AUTHORS   Okamoto S, Niki H.
  TITLE     pRIT21 vector sequence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7064)
  AUTHORS   Okamoto S, Niki H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2016) Contact:Sho Okamoto National Institute of
            Genetics, Microbial Genetics Laboratory, Genetics Strains Reseach
            Center; 1111 Yata, Mishima, Shizuoka 411-8540, Japan URL
            :http://www.nig.ac.jp/labs/MicroGen/index.html
REFERENCE   3  (bases 1 to 7064)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7064)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (26-JAN-2016) Contact:Sho Okamoto National Institute of Genetics,
            Microbial Genetics Laboratory, Genetics Strains Reseach Center; 1111
            Yata, Mishima, Shizuoka 411-8540, Japan URL
            :http://www.nig.ac.jp/labs/MicroGen/index.html"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7064
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1260..1907
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             complement(2663..2677)
                     /label="enterokinase site"
                     /note="enterokinase recognition and cleavage site"
     CDS             3773..3943
                     /label="ProtA"
                     /note="IgG-binding unit of Staphylococcus aureus protein A"
     misc_feature    4469..4524
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     promoter        4998..5102
                     /label="AmpR promoter"
     CDS             5103..5960
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6134..6722
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"