prCol2(P)-FRT vector (V003822)

Basic Vector Information

      • Vector Name:
      • prCol2(P)-FRT
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 4899 bp
      • Type:
      • Expression vector
      • Replication origin:
      • ori
      • Source/Author:
      • Ito K, Shinomura T.
      • Promoter:
      • SP6

prCol2(P)-FRT vector Vector Map

prCol2(P)-FRT4899 bp6001200180024003000360042004800SP6 promoterCol2a1FRTT7 promoterM13 fwdf1 oriAmpR promoterAmpRoriCAP binding sitelac promoterlac operatorM13 rev

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

prCol2(P)-FRT vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_36408        4899 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector prCol2(P)-FRT DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4899)
  AUTHORS   Ito K, Shinomura T.
  TITLE     Development and application of a new Silent reporter system to 
            quantitate the activity of enhancer elements in the type II Collagen
            Gene
  JOURNAL   Gene (2016) In press
  PUBMED    26992640
REFERENCE   2  (bases 1 to 4899)
  AUTHORS   Shinomura T.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2015) Contact:Tamayuki Shinomura Tokyo Medical and
            Dental University, Tissue Regeneration; 1-5-45 Yushima, Bunkyo-ku, 
            Tokyo 113-8549, Japan
REFERENCE   3  (bases 1 to 4899)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4899)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene (2016)
            In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-OCT-2015) Contact:Tamayuki Shinomura Tokyo Medical and Dental 
            University, Tissue Regeneration; 1-5-45 Yushima, Bunkyo-ku, Tokyo 
            113-8549, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4899
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        14..32
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     gene            78..1727
                     /gene="Col2a1"
                     /label=Col2a1
     regulatory      78..1066
                     /gene="Col2a1"
                     /label=rat type II collagen
                     /note="rat type II collagen"
                     /regulatory_class="promoter"
     exon            1067..1307
                     /gene="Col2a1"
                     /number=1
                     /note="rat type II collagen"
     intron          1308..1351
                     /gene="Col2a1"
                     /number=1
                     /note="rat type II collagen partial intron"
     intron          1556..1710
                     /gene="Col2a1"
                     /number=2
                     /note="rat type II collagen partial intron"
     exon            1711..1727
                     /gene="Col2a1"
                     /number=3
                     /note="rat type II collagen partial exon"
     protein_bind    1736..1783
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        complement(1798..1816)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1823..1839)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(1980..2435)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2769..2873
                     /label=AmpR promoter
     CDS             2874..3731
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3905..4493
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4781..4802
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4817..4847
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4855..4871
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4879..4895
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"

This page is informational only.