pR6Kamp-LAP(GFP) vector (V003839)

LOCUS       40924_36288        4232 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pR6Kamp-LAP(GFP), complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Poser I, Sarov M, Hutchins JR, Heriche JK, Toyoda Y, Pozniakovsky A,
            Weigl D, Nitzsche A, Hegemann B, Bird AW, Pelletier L, Kittler R, 
            Hua S, Naumann R, Augsburg M, Sykora MM, Hofemeister H, Zhang Y, 
            Nasmyth K, White KP, Dietzel S, Mechtler K, Durbin R, Stewart AF, 
            Peters JM, Buchholz F, Hyman AA.
  TITLE     BAC TransgeneOmics: a high-throughput method for exploration of 
            protein function in mammals
  JOURNAL   Nat. Methods 5 (5), 409-415 (2008)
  PUBMED    18391959
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   Hyman AA.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) Human Genetics, University of Chicago, 900 E
            57th St, Kcbd 10240, Chicago, IL 60605, USA
REFERENCE   3  (bases 1 to 4232)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4232)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. 
            Methods"; date: "2008"; volume: "5"; issue: "5"; pages: "409-415"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-APR-2018) Human Genetics, University of Chicago, 900 E 57th St, 
            Kcbd 10240, Chicago, IL 60605, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4232
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..27
                     /label=forward recombineering primer sequence
                     /note="forward recombineering primer sequence"
     CDS             28..48
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     misc_feature    61..105
                     /label=S-peptide
                     /note="S-peptide"
     CDS             61..105
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             145..168
                     /label=HRV 3C site
                     /note="recognition and cleavage site for human rhinovirus
                     3C and PreScission proteases"
     CDS             196..912
                     /label=EGFP
                     /note="enhanced GFP"
     misc_feature    953..1538
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     regulatory      1541..1546
                     /regulatory_class="minus_35_signal"
     regulatory      1561..1569
                     /regulatory_class="minus_10_signal"
     CDS             1620..2420
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
     oriT            complement(2457..2566)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      2725..3113
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     promoter        3207..3311
                     /label=AmpR promoter
     CDS             3312..4169
                     /label=AmpR
                     /note="beta-lactamase"