pR6K-lys-Gm vector (V003840)

LOCUS       V003840                 7163 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003840
VERSION     V003840
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7163)
  AUTHORS   Kang Y, Son MS, Hoang TT.
  TITLE     One step engineering of T7-expression strains for protein
            production: increasing the host-range of the T7-expression system
  JOURNAL   Protein Expr. Purif. 55 (2), 325-333 (2007)
   PUBMED   17716915
REFERENCE   2  (bases 1 to 7163)
  AUTHORS   Kang Y, Son MS, Hoang TT.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-NOV-2006) Microbiology, University of Hawaii at Manoa,
            2538 The Mall - Snyder 310, Honolulu, HI 96822, USA
REFERENCE   3  (bases 1 to 7163)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7163)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein
            Expr. Purif."; date: "2007"; volume: "55"; issue: "2"; pages:
            "325-333"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (30-NOV-2006) Microbiology, University of Hawaii at Manoa, 2538 The
            Mall - Snyder 310, Honolulu, HI 96822, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7163
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(7..25)
                     /label="Tn5 ME"
                     /note="hyperactive mosaic end for Tn5 transposase
                     recognition (Reznikoff et al., 2004)"
     protein_bind    37..268
                     /label="lambda attP"
                     /note="integrase from phage lambda"
     rep_origin      complement(561..946)
                     /direction=LEFT
                     /label="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     misc_feature    951..969
                     /label="Tn5 ME"
                     /note="hyperactive mosaic end for Tn5 transposase
                     recognition (Reznikoff et al., 2004)"
     CDS             complement(981..3629)
                     /note="T7 RNA polymerase from Escherichia phage T7.
                     Accession#: P00573"
                     /label="T7 RNA polymerase"
     CDS             complement(3938..4111)
                     /label="lacZ-alpha"
                     /note="LacZ-alpha fragment of beta-galactosidase"
     protein_bind    complement(4131..4147)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4155..4185)
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    complement(4200..4221)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(4237..5316)
                     /label="lacI"
                     /note="lac repressor"
     misc_feature    complement(5397..5444)
                     /label="Flp-recombination-target"
                     /note="Flp-recombination-target"
     protein_bind    complement(5397..5444)
                     /label="FRT"
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        5485..5513
                     /label="Pc promoter"
                     /note="class 1 integron promoter"
     CDS             5702..6232
                     /label="GmR"
                     /note="gentamycin acetyltransferase"
     protein_bind    complement(6365..6412)
                     /label="FRT"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6675..7127
                     /label="T7 lysozyme"
                     /note="lysozyme from bacteriophage T7"