pQLinkHD vector (V003850)

LOCUS       40924_36228        7062 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pQLinkHD, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7062)
  AUTHORS   Scheich C, Kummel D, Soumailakakis D, Heinemann U, Bussow K.
  TITLE     Vectors for co-expression of an unrestricted number of proteins
  JOURNAL   Nucleic Acids Res. 35 (6), E43 (2007)
  PUBMED    17311810
REFERENCE   2  (bases 1 to 7062)
  AUTHORS   Buessow K, Scheich C.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-SEP-2006) Vertebrate Genomics, Max Planck Institute 
            for Molecular Genetics, Ihnestr. 63-73, Berlin 14195, Germany
REFERENCE   3  (bases 1 to 7062)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7062)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2007"; volume: "35"; issue: "6"; pages: "E43"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-SEP-2006) Vertebrate Genomics, Max Planck Institute for 
            Molecular Genetics, Ihnestr. 63-73, Berlin 14195, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7062
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    7..27
                     /label=LIC cassette with PacI restriction site
                     /note="LIC cassette with PacI restriction site"
     promoter        36..80
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    88..104
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             123..134
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     regulatory      127..133
                     /regulatory_class="ribosome_binding_site"
     misc_feature    141
                     /note="translation start site for inserts cloned into the 
                     multiple cloning site"
     CDS             147..167
                     /label=7xHis
                     /note="6xHis affinity tag"
     protein_bind    180..304
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(736..1038)
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             complement(1386..2042)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(2043..2145)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     protein_bind    complement(2323..2447)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      2469..2563
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     misc_feature    2568..2617
                     /label=LIC cassette with SwaI, PacI restriction sites
                     /note="LIC cassette with SwaI, PacI restriction sites"
     CDS             2657..3313
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      3381..3467
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    complement(3526..3547)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(3563..4642)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(4643..4720)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     misc_feature    4912..5052
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      5236
                     /label=ColE1 ori
                     /note="ColE1 ori"
     rep_origin      complement(5238..5826)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6000..6857)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6858..6962)
                     /label=AmpR promoter