pPxylA+-cel8A vector (V003887)

LOCUS       40924_35718        8084 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pPxylA+-cel8A, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8084)
  AUTHORS   Heinze S, Zimmermann K, Ludwig C, Heinzlmeir S, Schwarz WH, Zverlov 
            VV, Liebl W, Kornberger P.
  TITLE     Efficient secretory heterologous enzyme production in the novel host
            Paenibacillus polymyxa by usage of its promoter sequences
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8084)
  AUTHORS   Heinze S, Zimmermann K, Ludwig C, Heinzlmeir S, Schwarz WH, Zverlov 
            VV, Liebl W, Kornberger P.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAY-2018) Department for Microbiology, Technical 
            University of Munich, Emil-Ramann-Str. 4, Freising, Bavaria 85354, 
            Germany
REFERENCE   3  (bases 1 to 8084)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8084)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-MAY-2018) Department for Microbiology, Technical University of 
            Munich, Emil-Ramann-Str. 4, Freising, Bavaria 85354, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8084
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(79..667)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(841..1698)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(1699..1803)
                     /label=AmpR promoter
     CDS             complement(1832..2116)
                     /codon_start=1
                     /gene="traJ"
                     /product="TraJ"
                     /label=traJ
                     /protein_id="AXF54489.1"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGQQKAHYVNHHPNQVFWGRGAVKH"
     gene            complement(1832..2116)
                     /gene="traJ"
                     /label=traJ
     oriT            complement(2149..2258)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(2515..3276)
                     /codon_start=1
                     /gene="kanR"
                     /product="KanR"
                     /label=kanR
                     /note="Kanamycin Resistance"
                     /protein_id="AXF54490.1"
                     /translation="MNGPIIMTREERMKIVHEIKERILDKYGDDVKAIGVYGSLGRQTD
                     GPYSDIEMMCVMSTEEAEFSHEWTTGEWKVEVNFDSEEILLDYASQVESDWPLTHGQFF
                     SILPIYDSGGYLEKVYQTAKSVEAQTFHDAICALIVEELFEYAGKWRNIRVQGPTTFLP
                     SLTVQVAMAGAMLIGLHHRICYTTSASVLTEAVKQSDLPSGYDHLCQFVMSGQLSDSEK
                     LLESLENFWNGIQEWTERHGYIVDVSKRIPF"
     gene            complement(2515..3276)
                     /gene="kanR"
                     /label=kanR
     CDS             complement(3448..4449)
                     /label=repB
                     /note="RepB replication protein"
     CDS             complement(5063..6229)
                     /codon_start=1
                     /gene="xylR"
                     /product="XylR"
                     /label=xylR
                     /note="XylR from B. megaterium xyl-operon"
                     /protein_id="AXF54491.1"
                     /translation="MVIIQIADQALVKKMNQKLILDEILKNSPVSRATLSEITGLNKST
                     VSSQVNTLLEKDFIFEIGAGQSRGGRRPVMLVFNKNAGYSIGIDIGVDYLNGILTDLEG
                     NIILEKTSDLSSSSASEVKEILFALIHGFVTHMPESPYGLVGIGICVPGLVDRHQQIIF
                     MPNLNWNIKDLQFLIESEFNVPVFVENEANAGAYGEKVFGMTKNYENIVYISINIGIGT
                     GLVINNELYKGVQGFSGEMGHMTIDFNGPKCSCGNRGCWELYASEKALLASLSKEEKNI
                     SRKEIVERANKNDVEMLNALQNFGFYIGIGLTNILNTFDIEAVILRNHIIESHPIVLNT
                     IKNEVSSRVHSHLDNKCELLPSSLGKNAPALGAVSIVIDSFLSVTPIS"
     gene            complement(5063..6229)
                     /gene="xylR"
                     /label=xylR
     regulatory      6333..6367
                     /note="PxylA, xylose inducible promoter, derived from
                     pHIS1525"
                     /regulatory_class="promoter"
     misc_feature    6369..6397
                     /label=XylR binding site
                     /note="XylR binding site"
     regulatory      6438..6447
                     /note="rbs+, ribosome binding site optimized for B.
                     megaterium"
                     /regulatory_class="ribosome_binding_site"
     CDS             6461..6544
                     /codon_start=1
                     /gene="SPLipB-Cel8A-His6"
                     /product="LipB signal peptide"
                     /label=SPLipB-Cel8A-His6
                     /protein_id="AXF54492.1"
                     /translation="MKKVLMAFIICLSLILSVLAAPPSGAKA"
     CDS             7904..7921
                     /label=6xHis
                     /note="6xHis affinity tag"