pPW380 vector (V003889)

LOCUS       40924_35708        9219 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pPW380, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9219)
  AUTHORS   Woo PC, Ma SS, Teng JL, Li MW, Kao RY, Lau SK, Yuen KY.
  TITLE     Construction of an inducible expression shuttle vector for 
            Laribacter hongkongensis, a novel bacterium associated with 
            gastroenteritis
  JOURNAL   FEMS Microbiol. Lett. 252 (1), 57-65 (2005)
  PUBMED    16165318
REFERENCE   2  (bases 1 to 9219)
  AUTHORS   Woo PCY., Ma SSL., Teng JLL., Li MWS., Lau SKP., Yuen KY.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-AUG-2005) Microbiology, The University of Hong Kong, 
            Hong Kong
REFERENCE   3  (bases 1 to 9219)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9219)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS 
            Microbiol. Lett."; date: "2005"; volume: "252"; issue: "1"; pages: 
            "57-65"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-AUG-2005) Microbiology, The University of Hong Kong, Hong Kong"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9219
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      7..462
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    complement(469..590)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     952..968
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        975..993
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1981..2604
                     /codon_start=1
                     /gene="parA"
                     /product="ATPase"
                     /label=parA
                     /note="involved in chromosome partitioning"
                     /protein_id="AAZ91679.1"
                     /translation="MRTIVVASQKGGVGKTTIAGHLGVMAEQSKEGPVALIDTDPQGSL
                     ASWWNERTDEAPLFARVEINKLTEHLQALAKGGIKLAIIDTPPSVTEMIQQVLRTADLV
                     LIPTRPSPHDLRAVGSTVELVENAGKRMIFVINGAAPRARIAGEAAVALSQHGTVAPVT
                     LYQRTDFASSMIDGRTVQEIDPKGRSAEEIGKLWKYVSTQLRKI"
     gene            1981..2604
                     /gene="parA"
                     /label=parA
     CDS             3058..3975
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein"
                     /label=repA
                     /note="plasmid replication initiator protein"
                     /protein_id="AAZ91680.1"
                     /translation="MAEVVDEQPARRKRTPKGPRGLMPVHHPNRDFFLCDLFNYALKDD
                     GVSMEAPIFTLATKPDTSVWHWESKDGARAITVTPSVKGRATQFDKDLLIYVVSQMTEA
                     MNRGRPDANSRTVRFRVYDYLVSTNKPTGGKEYQRLEDALDRLRGTSIKTNIKTGGQRV
                     KEGFGIVDSWTIIEKAPDDDRMIAVEVTLSKWLFNAVQAHEVLTINPDYFRLRKPIERR
                     LYELARKHCGDQAFFVIGLELLQDKCGSKSALFEFRRALREIIKADTLPDYRMTLDNEK
                     DQVIFYTRDAKKLTASAALPQRFQ"
     gene            3058..3975
                     /gene="repA"
                     /label=repA
     promoter        complement(5841..5859)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5880..5896)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5904..5920)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6029..6232)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(6233..6536)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(6711..7299)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    complement(7628..7675)
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(7910..8701)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(8736..9093)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        complement(9095..9199)
                     /label=AmpR promoter