pPTbsr vector (V003908)

LOCUS       V003908                 4168 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003908
VERSION     V003908
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Buck JM.
  TITLE     Blasticidin-S deaminase, a new selection marker for genetic
            transformation of the diatom Phaeodactylum tricornutum
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4168)
  AUTHORS   Buck JM.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2018) Department of Biology, University of
            Konstanz, Universitatsstrasse 10, Konstanz 78457, Germany
REFERENCE   3  (bases 1 to 4168)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4168)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (26-JUN-2018) Department of Biology, University of Konstanz,
            Universitatsstrasse 10, Konstanz 78457, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4168
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    461..517
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     CDS             1105..1524
                     /gene="bsr"
                     /label="Blasticidin-S deaminase"
                     /note="Blasticidin-S deaminase from Bacillus cereus.
                     Accession#: P33967"
     promoter        complement(1792..1810)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2068..2656)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2830..3687)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3688..3792)
                     /label="AmpR promoter"
     promoter        4138..4156
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"