pPpGUT1 vector (V003941)

LOCUS       40924_35409        7107 bp DNA     circular SYN 18-DEC-2018
DEFINITION  E.coli-K.pastoris shuttle vector pPpGUT1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7107)
  AUTHORS   Naatsaari L, Mistlberger B, Ruth C, Hajek T, Hartner FS, Glieder A.
  TITLE     Deletion of the Pichia pastoris KU70 Homologue Facilitates Platform 
            Strain Generation for Gene Expression and Synthetic Biology
  JOURNAL   PLoS ONE 7 (6), E39720 (2012)
  PUBMED    22768112
REFERENCE   2  (bases 1 to 7107)
  AUTHORS   Naeaetsaari L, Mistlberger B, Ruth C, Hajek T, Hartner F, Glieder A.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2012) Institute of Molecular Biotechnology, Graz 
            University of Technology, Petersgasse 14/2, Graz, Styria 8010, 
            Austria
REFERENCE   3  (bases 1 to 7107)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7107)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2012"; volume: "7"; issue: "6"; pages: "E39720"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-JAN-2012) Institute of Molecular Biotechnology, Graz University 
            of Technology, Petersgasse 14/2, Graz, Styria 8010, Austria"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: SeqMan v. 7.0.0
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7107
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..900
                     /label=GUT1 promoter
                     /note="GUT1 promoter"
                     /regulatory_class="promoter"
     CDS             901..2766
                     /codon_start=1
                     /product="glycerol kinase"
                     /label=glycerol kinase
                     /note="GUT1"
                     /protein_id="AFJ20664.1"
                     /translation="MGKDYTPLVATIDIGTTSTRAILFDYHGQEVAKHQIEYSTSAQDD
                     IKRKRSQIISSEGISLTVSDDLEVESVDNKAGPTLQFPQPGWVECRPSHILANAVQCLA
                     ACLVTMENKNLDRDEKNKYKLISIGVANMRETTVVWSKKTGKPLYNGIVWNDTRNNDIV
                     DEYTAKYSEKEREEMRTLCGCPISTYFSATKFRWLLKHVPEVKQAYDNADGDLMFGTID
                     SWLIYHLTNEKSHVTDVTNASRTNFMNIETNKYDDRLLKFWDVDTSKVILPEIRSSAEV
                     YGHFKVPHLESIGYVESYLTDDALALLETIEGAPLAGCLGDQSASLVGQLAVRKGDAKC
                     TYGTGAFLLYNTGDQTLISEHGALTTVGYWFPGLDESEDGKHSSKPQYALEGSIAVAGS
                     VVQWLRDNLRLISKAQDVGPLASQVDNSGGVVFVPAFSGLFAPYWDSNSRGTIFGLTQY
                     TSASHIARAALEGVCFQTRAILKAMISDAGASADFLEESSKATGHNPLSVLAVDGGMSK
                     SDEMMQIQADILGPCVTVRRSINPECTALGAAIAAGFGVPKEDRIWGSLKECTEAILEG
                     NKMYLAAGNTSLDFKATLSDEVRRKEWRLWENAIAKAKGWLKDTA"
     regulatory      2767..3266
                     /label=GUT1 terminator
                     /note="GUT1 terminator"
                     /regulatory_class="terminator"
     promoter        3273..4204
                     /label=AOX1 promoter
                     /note="inducible promoter, regulated by methanol"
     terminator      4232..4478
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     misc_feature    4492..5184
                     /note="integration sequence for recombination into target
                     locus"
     regulatory      5185..5242
                     /label=EM72 synthetic promoter
                     /note="EM72 synthetic promoter"
                     /regulatory_class="promoter"
     CDS             5243..6100
                     /label=AmpR
                     /note="beta-lactamase"
     terminator      6110..6297
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     rep_origin      6461..7049
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"