8xNFAT-ZsG-hCD8 vector (V011720)

Price Information

Cat No. Plasmid Name Availability Add to cart
V011720 8xNFAT-ZsG-hCD8 In stock, 1 week for quality controls

Buy one, get one free!

Two tubes of lyophilized plasmid will be delivered, each tube is about 5µg.

Basic Vector Information

Vector Name:
8xNFAT-ZsG-hCD8
Antibiotic Resistance:
Ampicillin
Length:
7850 bp
Type:
Mammalian Expression, Retroviral
Replication origin:
ori
Copy Number:
High Copy
Promoter:
mPGK
Cloning Method:
Gibson Cloning
5' Primer:
NA

8xNFAT-ZsG-hCD8 vector Vector Map

8xNFAT-ZsG-hCD87850 bp30060090012001500180021002400270030003300360039004200450048005100540057006000630066006900720075007800CMV enhancerCMV promoter5' LTR (truncated)MMLV Psigag (truncated)NFAT binding siteNFAT binding siteNFAT binding siteNFAT binding siteNFAT binding siteNFAT binding siteNFAT binding siteNFAT binding siteminPZsGreen1PGK promoterT-cell surface glycoprotein CD8 alpha chainP2AT-cell surface glycoprotein CD8 beta chain3' LTR (Delta-U3)oriAmpRAmpR promoterpBRforEcopGEX 3'pRS-markerIn lacZ genepBRrevBam

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

8xNFAT-ZsG-hCD8 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       V011720                 7850 bp    DNA     circular SYN 13-MAY-2021
DEFINITION  Exported.
ACCESSION   V011720
VERSION     V011720
KEYWORDS    8xNFAT-ZsG-hCD8.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7850)
  AUTHORS   Mann SE, Zhou Z, Landry LG, Anderson AM, Alkanani AK, Fischer J,
            Peakman M, Mallone R, Campbell K, Michels AW, Nakayama M
  TITLE     Multiplex T Cell Stimulation Assay Utilizing a T Cell Activation
            Reporter-Based Detection System.
  JOURNAL   Front Immunol. 2020 Apr 9;11:633. doi: 10.3389/fimmu.2020.00633.
            eCollection 2020.
   PUBMED   32328071
REFERENCE   2  (bases 1 to 7850)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7850)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front
            Immunol."; date: "2020-04-9"; pages: "
            10.3389/fimmu.2020.00633. eCollection 2020"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7850
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             585..760
                     /label="5' LTR (truncated)"
                     /note="truncated long terminal repeat from Moloney murine
                     sarcoma virus"
     misc_feature    823..1022
                     /label="MMLV Psi"
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             1223..1639
                     /label="gag (truncated)"
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
     protein_bind    1673..1702
                     /label="NFAT binding site"
                     /note="NFAT binding site from the human interleukin 2
                     (IL-2) gene (Mattila et al., 1990)"
     protein_bind    1703..1732
                     /label="NFAT binding site"
                     /bound_moiety="NFAT transcription factor"
                     /note="NFAT binding site from the human interleukin 2
                     (IL-2) gene (Mattila et al., 1990)"
     protein_bind    1733..1762
                     /label="NFAT binding site"
                     /bound_moiety="NFAT transcription factor"
                     /note="NFAT binding site from the human interleukin 2
                     (IL-2) gene (Mattila et al., 1990)"
     protein_bind    1763..1792
                     /label="NFAT binding site"
                     /bound_moiety="NFAT transcription factor"
                     /note="NFAT binding site from the human interleukin 2
                     (IL-2) gene (Mattila et al., 1990)"
     protein_bind    1793..1822
                     /label="NFAT binding site"
                     /bound_moiety="NFAT transcription factor"
                     /note="NFAT binding site from the human interleukin 2
                     (IL-2) gene (Mattila et al., 1990)"
     protein_bind    1823..1852
                     /label="NFAT binding site"
                     /bound_moiety="NFAT transcription factor"
                     /note="NFAT binding site from the human interleukin 2
                     (IL-2) gene (Mattila et al., 1990)"
     protein_bind    1853..1882
                     /label="NFAT binding site"
                     /bound_moiety="NFAT transcription factor"
                     /note="NFAT binding site from the human interleukin 2
                     (IL-2) gene (Mattila et al., 1990)"
     protein_bind    1883..1912
                     /label="NFAT binding site"
                     /bound_moiety="NFAT transcription factor"
                     /note="NFAT binding site from the human interleukin 2
                     (IL-2) gene (Mattila et al., 1990)"
     promoter        1924..1955
                     /label="minP"
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             1975..2667
                     /label="ZsGreen1"
                     /note="Zoanthus green fluorescent protein"
     promoter        2693..3192
                     /label="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             3210..3914
                     /gene="CD8A"
                     /label="T-cell surface glycoprotein CD8 alpha chain"
                     /note="T-cell surface glycoprotein CD8 alpha chain from
                     Homo sapiens. Accession#: P01732"
     CDS             3924..3980
                     /label="P2A"
                     /note="2A peptide from porcine teschovirus-1 polyprotein"
     CDS             3981..4601
                     /gene="CD8B"
                     /label="T-cell surface glycoprotein CD8 beta chain"
                     /note="T-cell surface glycoprotein CD8 beta chain from Homo
                     sapiens. Accession#: P10966"
     LTR             4793..5218
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     rep_origin      complement(5388..5976)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6150..7007)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7008..7112)
                     /label="AmpR promoter"
     primer_bind     7180..7198
                     /label="pBRforEco"
                     /note="pBR322 vectors, upsteam of EcoRI site, forward
                     primer"
     primer_bind     complement(7236..7258)
                     /label="pGEX 3'"
                     /note="pGEX vectors, reverse primer"
     primer_bind     7358..7377
                     /label="pRS-marker"
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
     primer_bind     7571..7593
                     /label="M13/pUC Forward"
                     /note="In lacZ gene"
     primer_bind     7721..7740
                     /label="pBRrevBam"
                     /note="pBR322 vectors, tet region, downstream of BamHI,
                     reverse primer"